Selection and analysis of spontaneous reciprocal mitotic cross-overs in Saccharomyces cerevisiae.

Published

Journal Article

We developed a system that allows the selection of the reciprocal products resulting from spontaneous mitotic cross-overs in the yeast Saccharomyces cerevisiae. A number of other types of genetic events, including chromosome loss, can be monitored with this system. For a 120-kb chromosome interval on chromosome V (CEN5-CAN1), the rate of mitotic cross-overs was 4x10(-5) per division, a rate approximately 25,000-fold lower than the meiotic rate of cross-overs. We found no suppression of mitotic cross-overs near the centromere of chromosome V, unlike the suppression observed for meiotic exchanges. The rate of reciprocal cross-overs was substantially (38-fold) elevated by treatment of cells with hydroxyurea, a drug that reduces nucleotide pools and slows DNA replication.

Full Text

Duke Authors

Cited Authors

  • Barbera, MA; Petes, TD

Published Date

  • August 14, 2006

Published In

Volume / Issue

  • 103 / 34

Start / End Page

  • 12819 - 12824

PubMed ID

  • 16908833

Pubmed Central ID

  • 16908833

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0605778103

Language

  • eng