Chromosomal translocations in yeast induced by low levels of DNA polymerase a model for chromosome fragile sites.

Published

Journal Article

In the yeast Saccharomyces cerevisiae, reduced levels of the replicative alpha DNA polymerase result in greatly elevated frequencies of chromosome translocations and chromosome loss. We selected translocations in a small region of chromosome III and found that they involve homologous recombination events between yeast retrotransposons (Ty elements) on chromosome III and retrotransposons located on other chromosomes. One of the two preferred sites of these translocations on chromosome III involve two Ty elements arrayed head-to-head; disruption of this site substantially reduces the rate of translocations. We demonstrate that this pair of Ty elements constitutes a preferred site for double-strand DNA breaks when DNA replication is compromised, analogous to the fragile sites observed in mammalian chromosomes.

Full Text

Duke Authors

Cited Authors

  • Lemoine, FJ; Degtyareva, NP; Lobachev, K; Petes, TD

Published Date

  • March 2005

Published In

Volume / Issue

  • 120 / 5

Start / End Page

  • 587 - 598

PubMed ID

  • 15766523

Pubmed Central ID

  • 15766523

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2004.12.039

Language

  • eng