Caenorhabditis elegans DNA mismatch repair gene msh-2 is required for microsatellite stability and maintenance of genome integrity.

Published

Journal Article

Mismatch repair genes are important in maintaining the fidelity of DNA replication. To determine the function of the Caenorhabditis elegans homologue of the MSH2 mismatch repair gene (msh-2), we isolated a strain of C. elegans with an insertion of the transposable element Tc1 within msh-2. Early-passage msh-2 mutants were similar to wild-type worms with regard to lifespan and meiotic chromosome segregation but had slightly reduced fertility. The mutant worms had reduced DNA damage-induced germ-line apoptosis after genotoxic stress. The msh-2 mutants also had elevated levels of microsatellite instability and increased rates of reversion of the dominant unc-58(e665) mutation. In addition, serially passaged cultures of msh-2 worms died out much more quickly than those of wild-type worms. These results demonstrate that msh-2 function in C. elegans is important in regulating both short- and long-term genomic stability.

Full Text

Duke Authors

Cited Authors

  • Degtyareva, NP; Greenwell, P; Hofmann, ER; Hengartner, MO; Zhang, L; Culotti, JG; Petes, TD

Published Date

  • February 19, 2002

Published In

Volume / Issue

  • 99 / 4

Start / End Page

  • 2158 - 2163

PubMed ID

  • 11830642

Pubmed Central ID

  • 11830642

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.032671599

Language

  • eng

Conference Location

  • United States