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Caenorhabditis elegans DNA mismatch repair gene msh-2 is required for microsatellite stability and maintenance of genome integrity.

Publication ,  Journal Article
Degtyareva, NP; Greenwell, P; Hofmann, ER; Hengartner, MO; Zhang, L; Culotti, JG; Petes, TD
Published in: Proc Natl Acad Sci U S A
February 19, 2002

Mismatch repair genes are important in maintaining the fidelity of DNA replication. To determine the function of the Caenorhabditis elegans homologue of the MSH2 mismatch repair gene (msh-2), we isolated a strain of C. elegans with an insertion of the transposable element Tc1 within msh-2. Early-passage msh-2 mutants were similar to wild-type worms with regard to lifespan and meiotic chromosome segregation but had slightly reduced fertility. The mutant worms had reduced DNA damage-induced germ-line apoptosis after genotoxic stress. The msh-2 mutants also had elevated levels of microsatellite instability and increased rates of reversion of the dominant unc-58(e665) mutation. In addition, serially passaged cultures of msh-2 worms died out much more quickly than those of wild-type worms. These results demonstrate that msh-2 function in C. elegans is important in regulating both short- and long-term genomic stability.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

February 19, 2002

Volume

99

Issue

4

Start / End Page

2158 / 2163

Location

United States

Related Subject Headings

  • Time Factors
  • Proto-Oncogene Proteins
  • Mutation
  • MutS Homolog 2 Protein
  • MutS DNA Mismatch-Binding Protein
  • Molecular Sequence Data
  • Models, Genetic
  • Microsatellite Repeats
  • Meiosis
  • Humans
 

Citation

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Degtyareva, N. P., Greenwell, P., Hofmann, E. R., Hengartner, M. O., Zhang, L., Culotti, J. G., & Petes, T. D. (2002). Caenorhabditis elegans DNA mismatch repair gene msh-2 is required for microsatellite stability and maintenance of genome integrity. Proc Natl Acad Sci U S A, 99(4), 2158–2163. https://doi.org/10.1073/pnas.032671599
Degtyareva, Natasha P., Patricia Greenwell, E Randal Hofmann, Michael O. Hengartner, Lijia Zhang, Joseph G. Culotti, and Thomas D. Petes. “Caenorhabditis elegans DNA mismatch repair gene msh-2 is required for microsatellite stability and maintenance of genome integrity.Proc Natl Acad Sci U S A 99, no. 4 (February 19, 2002): 2158–63. https://doi.org/10.1073/pnas.032671599.
Degtyareva NP, Greenwell P, Hofmann ER, Hengartner MO, Zhang L, Culotti JG, et al. Caenorhabditis elegans DNA mismatch repair gene msh-2 is required for microsatellite stability and maintenance of genome integrity. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2158–63.
Degtyareva, Natasha P., et al. “Caenorhabditis elegans DNA mismatch repair gene msh-2 is required for microsatellite stability and maintenance of genome integrity.Proc Natl Acad Sci U S A, vol. 99, no. 4, Feb. 2002, pp. 2158–63. Pubmed, doi:10.1073/pnas.032671599.
Degtyareva NP, Greenwell P, Hofmann ER, Hengartner MO, Zhang L, Culotti JG, Petes TD. Caenorhabditis elegans DNA mismatch repair gene msh-2 is required for microsatellite stability and maintenance of genome integrity. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2158–2163.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

February 19, 2002

Volume

99

Issue

4

Start / End Page

2158 / 2163

Location

United States

Related Subject Headings

  • Time Factors
  • Proto-Oncogene Proteins
  • Mutation
  • MutS Homolog 2 Protein
  • MutS DNA Mismatch-Binding Protein
  • Molecular Sequence Data
  • Models, Genetic
  • Microsatellite Repeats
  • Meiosis
  • Humans