Regulation of genome stability by TEL1 and MEC1, yeast homologs of the mammalian ATM and ATR genes.

Journal Article (Journal Article)

In eukaryotes, a family of related protein kinases (the ATM family) is involved in regulating cellular responses to DNA damage and telomere length. In the yeast Saccharomyces cerevisiae, two members of this family, TEL1 and MEC1, have functionally redundant roles in both DNA damage repair and telomere length regulation. Strains with mutations in both genes are very sensitive to DNA damaging agents, have very short telomeres, and undergo cellular senescence. We find that strains with the double mutant genotype also have approximately 80-fold increased rates of mitotic recombination and chromosome loss. In addition, the tel1 mec1 strains have high rates of telomeric fusions, resulting in translocations, dicentrics, and circular chromosomes. Similar chromosome rearrangements have been detected in mammalian cells with mutations in ATM (related to TEL1) and ATR (related to MEC1) and in mammalian cells that approach cell crisis.

Full Text

Duke Authors

Cited Authors

  • Craven, RJ; Greenwell, PW; Dominska, M; Petes, TD

Published Date

  • June 2002

Published In

Volume / Issue

  • 161 / 2

Start / End Page

  • 493 - 507

PubMed ID

  • 12072449

Pubmed Central ID

  • PMC1462148

International Standard Serial Number (ISSN)

  • 0016-6731

Digital Object Identifier (DOI)

  • 10.1093/genetics/161.2.493


  • eng

Conference Location

  • United States