Isolation and characterization of point mutations in mismatch repair genes that destabilize microsatellites in yeast.

Journal Article (Journal Article)

The stability of simple repetitive DNA sequences (microsatellites) is a sensitive indicator of the ability of a cell to repair DNA mismatches. In a genetic screen for yeast mutants with elevated microsatellite instability, we identified strains containing point mutations in the yeast mismatch repair genes, MSH2, MSH3, MLH1, and PMS1. Some of these mutations conferred phenotypes significantly different from those of null mutations in these genes. One semidominant MSH2 mutation was identified. Finally we showed that strains heterozygous for null mutations of mismatch repair genes in diploid strains in yeast confer subtle defects in the repair of small DNA loops.

Full Text

Duke Authors

Cited Authors

  • Sia, EA; Dominska, M; Stefanovic, L; Petes, TD

Published Date

  • December 2001

Published In

Volume / Issue

  • 21 / 23

Start / End Page

  • 8157 - 8167

PubMed ID

  • 11689704

Pubmed Central ID

  • PMC99980

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/MCB.21.23.8157-8167.2001


  • eng

Conference Location

  • United States