Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae.

Journal Article (Journal Article)

In the yeast Saccharomyces cerevisiae, meiotic recombination is initiated by double-strand DNA breaks (DSBs). Meiotic DSBs occur at relatively high frequencies in some genomic regions (hotspots) and relatively low frequencies in others (coldspots). We used DNA microarrays to estimate variation in the level of nearby meiotic DSBs for all 6,200 yeast genes. Hotspots were nonrandomly associated with regions of high G + C base composition and certain transcriptional profiles. Coldspots were nonrandomly associated with the centromeres and telomeres.

Full Text

Duke Authors

Cited Authors

  • Gerton, JL; DeRisi, J; Shroff, R; Lichten, M; Brown, PO; Petes, TD

Published Date

  • October 10, 2000

Published In

Volume / Issue

  • 97 / 21

Start / End Page

  • 11383 - 11390

PubMed ID

  • 11027339

Pubmed Central ID

  • PMC17209

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.97.21.11383

Language

  • eng

Conference Location

  • United States