Global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae.
Journal Article (Journal Article)
In the yeast Saccharomyces cerevisiae, meiotic recombination is initiated by double-strand DNA breaks (DSBs). Meiotic DSBs occur at relatively high frequencies in some genomic regions (hotspots) and relatively low frequencies in others (coldspots). We used DNA microarrays to estimate variation in the level of nearby meiotic DSBs for all 6,200 yeast genes. Hotspots were nonrandomly associated with regions of high G + C base composition and certain transcriptional profiles. Coldspots were nonrandomly associated with the centromeres and telomeres.
Full Text
Duke Authors
Cited Authors
- Gerton, JL; DeRisi, J; Shroff, R; Lichten, M; Brown, PO; Petes, TD
Published Date
- October 10, 2000
Published In
Volume / Issue
- 97 / 21
Start / End Page
- 11383 - 11390
PubMed ID
- 11027339
Pubmed Central ID
- PMC17209
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.97.21.11383
Language
- eng
Conference Location
- United States