Analysis of microsatellite mutations in the mitochondrial DNA of Saccharomyces cerevisiae.

Published

Journal Article

In the nuclear genome of Saccharomyces cerevisiae, simple, repetitive DNA sequences (microsatellites) mutate at rates much higher than nonrepetitive sequences. Most of these mutations are deletions or additions of repeat units. The yeast mitochondrial genome also contains many microsatellites. To examine the stability of these sequences, we constructed a reporter gene (arg8(m)) containing out-of-frame insertions of either poly(AT) or poly(GT) tracts within the coding sequence. Yeast strains with this reporter gene inserted within the mitochondrial genome were constructed. Using these strains, we showed that poly(GT) tracts were considerably less stable than poly(AT) tracts and that alterations usually involved deletions rather than additions of repeat units. In contrast, in the nuclear genome, poly(GT) and poly(AT) tracts had similar stabilities, and alterations usually involved additions rather than deletions. Poly(GT) tracts were more stable in the mitochondria of diploid cells than in haploids. In addition, an msh1 mutation destabilized poly(GT) tracts in the mitochondrial genome.

Full Text

Duke Authors

Cited Authors

  • Sia, EA; Butler, CA; Dominska, M; Greenwell, P; Fox, TD; Petes, TD

Published Date

  • January 4, 2000

Published In

Volume / Issue

  • 97 / 1

Start / End Page

  • 250 - 255

PubMed ID

  • 10618404

Pubmed Central ID

  • 10618404

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.97.1.250

Language

  • eng

Conference Location

  • United States