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Dependence of the regulation of telomere length on the type of subtelomeric repeat in the yeast Saccharomyces cerevisiae.

Publication ,  Journal Article
Craven, RJ; Petes, TD
Published in: Genetics
August 1999

In the yeast Saccharomyces cerevisiae, chromosomes terminate with approximately 400 bp of a simple repeat poly(TG(1-3)). Based on the arrangement of subtelomeric X and Y' repeats, two types of yeast telomeres exist, those with both X and Y' (Y' telomeres) and those with only X (X telomeres). Mutations that result in abnormally short or abnormally long poly(TG(1-3)) tracts have been previously identified. In this study, we investigated telomere length in strains with two classes of mutations, one that resulted in short poly(TG(1-3)) tracts (tel1) and one that resulted in elongated tracts (pif1, rap1-17, rif1, or rif2). In the tel1 pif1 strain, Y' telomeres had about the same length as those in tel1 strains and X telomeres had lengths intermediate between those in tel1 and pif1 strains. Strains with either the tel1 rap1-17 or tel1 rif2 genotypes had short tracts for all chromosome ends examined, demonstrating that the telomere elongation characteristic of rap1-17 and rif2 strains is Tel1p-dependent. In strains of the tel1 rif1 or tel1 rif1 rif2 genotypes, telomeres with Y' repeats had short terminal tracts, whereas most of the X telomeres had long terminal tracts. These results demonstrate that the regulation of telomere length is different for X and Y' telomeres.

Duke Scholars

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

August 1999

Volume

152

Issue

4

Start / End Page

1531 / 1541

Location

United States

Related Subject Headings

  • Telomere-Binding Proteins
  • Telomere
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Repressor Proteins
  • Repetitive Sequences, Nucleic Acid
  • Protein Serine-Threonine Kinases
  • Intracellular Signaling Peptides and Proteins
  • Fungal Proteins
  • Epistasis, Genetic
 

Citation

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MLA
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Craven, R. J., & Petes, T. D. (1999). Dependence of the regulation of telomere length on the type of subtelomeric repeat in the yeast Saccharomyces cerevisiae. Genetics, 152(4), 1531–1541. https://doi.org/10.1093/genetics/152.4.1531
Craven, R. J., and T. D. Petes. “Dependence of the regulation of telomere length on the type of subtelomeric repeat in the yeast Saccharomyces cerevisiae.Genetics 152, no. 4 (August 1999): 1531–41. https://doi.org/10.1093/genetics/152.4.1531.
Craven, R. J., and T. D. Petes. “Dependence of the regulation of telomere length on the type of subtelomeric repeat in the yeast Saccharomyces cerevisiae.Genetics, vol. 152, no. 4, Aug. 1999, pp. 1531–41. Pubmed, doi:10.1093/genetics/152.4.1531.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

August 1999

Volume

152

Issue

4

Start / End Page

1531 / 1541

Location

United States

Related Subject Headings

  • Telomere-Binding Proteins
  • Telomere
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Repressor Proteins
  • Repetitive Sequences, Nucleic Acid
  • Protein Serine-Threonine Kinases
  • Intracellular Signaling Peptides and Proteins
  • Fungal Proteins
  • Epistasis, Genetic