A mutation of the yeast gene encoding PCNA destabilizes both microsatellite and minisatellite DNA sequences.

Published

Journal Article

The POL30 gene of the yeast Saccharomyces cerevisiae encodes the proliferating cell nuclear antigen (PCNA), a protein required for processive DNA synthesis by DNA polymerase delta and epsilon. We examined the effects of the pol30-52 mutation on the stability of microsatellite (1- to 8-bp repeat units) and minisatellite (20-bp repeat units) DNA sequences. It had previously been shown that this mutation destabilizes dinucleotide repeats 150-fold and that this effect is primarily due to defects in DNA mismatch repair. From our analysis of the effects of pol30-52 on classes of repetitive DNA with longer repeat unit lengths, we conclude that this mutation may also elevate the rate of DNA polymerase slippage. The effect of pol30-52 on tracts of repetitive DNA with large repeat unit lengths was similar, but not identical, to that observed previously for pol3-t, a temperature-sensitive mutation affecting DNA polymerase delta. Strains with both pol30-52 and pol3-t mutations grew extremely slowly and had minisatellite mutation rates considerably greater than those observed in either single mutant strain.

Full Text

Duke Authors

Cited Authors

  • Kokoska, RJ; Stefanovic, L; Buermeyer, AB; Liskay, RM; Petes, TD

Published Date

  • February 1999

Published In

Volume / Issue

  • 151 / 2

Start / End Page

  • 511 - 519

PubMed ID

  • 9927447

Pubmed Central ID

  • 9927447

Electronic International Standard Serial Number (EISSN)

  • 1943-2631

International Standard Serial Number (ISSN)

  • 0016-6731

Language

  • eng