Stabilization of microsatellite sequences by variant repeats in the yeast Saccharomyces cerevisiae.

Journal Article (Journal Article)

We examined the effect of a single variant repeat on the stability of a 51-base pair (bp) microsatellite (poly GT). We found that the insertion stabilizes the microsatellite about fivefold in wild-type strains. The stabilizing effect of the variant base was also observed in strains with mutations in the DNA mismatch repair genes pms1, msh2 and msh3, indicating that this effect does not require a functional DNA mismatch repair system. Most of the microsatellite alterations in the pms1, msh2 and msh3 strains were additions or deletions of single GT repeats, but about half of the alterations in the wild-type and msh6 strains were large (> 8 bp) deletions or additions.

Full Text

Duke Authors

Cited Authors

  • Petes, TD; Greenwell, PW; Dominska, M

Published Date

  • June 1997

Published In

Volume / Issue

  • 146 / 2

Start / End Page

  • 491 - 498

PubMed ID

  • 9178000

Pubmed Central ID

  • PMC1207991

International Standard Serial Number (ISSN)

  • 0016-6731

Digital Object Identifier (DOI)

  • 10.1093/genetics/146.2.491


  • eng

Conference Location

  • United States