Microsatellite instability in yeast: dependence on the length of the microsatellite.

Journal Article (Journal Article)

One of the most common microsatellites in eukaryotes consists of tandem arrays [usually 15-50 base pairs (bp) in length] of the dinucleotide GT. We examined the rates of instability for poly GT tracts of 15, 33, 51, 99 and 105 bp in wild-type and mismatch repair-deficient strains of Saccharomyces cerevisiae. Rates of instability increased more than two orders of magnitude as tracts increased in size from 15 to 99 bp in both wild-type and msh2 strains. The types of alterations observed in long and short tracts in wild-type strains were different in two ways. First, tracts > or = 51 bp had significantly more large deletions than tracts < or = 33 bp. Second, for the 99- and 105-bp tracts, almost all events involving single repeats were additions; for the smaller tracts, both additions and deletions of single repeats were common.

Full Text

Duke Authors

Cited Authors

  • Wierdl, M; Dominska, M; Petes, TD

Published Date

  • July 1997

Published In

Volume / Issue

  • 146 / 3

Start / End Page

  • 769 - 779

PubMed ID

  • 9215886

Pubmed Central ID

  • PMC1208050

International Standard Serial Number (ISSN)

  • 0016-6731

Digital Object Identifier (DOI)

  • 10.1093/genetics/146.3.769


  • eng

Conference Location

  • United States