Repair of DNA loops involves DNA-mismatch and nucleotide-excision repair proteins.

Published

Journal Article

A number of enzymes recognize and repair DNA lesions. The DNA-mismatch repair system corrects base-base mismatches and small loops, whereas the nucleotide-excision repair system removes pyrimidine dimers and other helix-distorting lesions. DNA molecules with mismatches or loops can arise as a consequence of heteroduplex formation during meiotic recombination. In the yeast Saccharomyces cerevisiae, repair of mismatches results in gene conversion or restoration, and failure to repair the mismatch results in post-meiotic segregation (PMS). The ratio of gene-conversion to PMS events reflects the efficiency of DNA repair. By examining the PMS patterns in yeast strains heterozygous for a mutant allele with a 26-base-pair insertion, we find that the repair of 26-base loops involves Msh2 (a DNA-mismatch repair protein) and Rad1 (a protein required for nucleotide-excision repair).

Full Text

Duke Authors

Cited Authors

  • Kirkpatrick, DT; Petes, TD

Published Date

  • June 26, 1997

Published In

Volume / Issue

  • 387 / 6636

Start / End Page

  • 929 - 931

PubMed ID

  • 9202128

Pubmed Central ID

  • 9202128

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/43225

Language

  • eng

Conference Location

  • England