Relationship between nuclease-hypersensitive sites and meiotic recombination hot spot activity at the HIS4 locus of Saccharomyces cerevisiae.
Journal Article (Journal Article)
Meiotic double-strand DNA breaks (DSBs), the lesions that initiate meiotic recombination at the HIS4 recombination hot spot, occur in a region upstream of the coding sequence associated with multiple DNase I-hypersensitive sites. Mutations in transcription factors that lead to loss of the DSBs result in the loss of some but not all DNase I-hypersensitive sites in the upstream region. A meiosis-specific change in chromatin structure is detected in strains with the wild-type hot spot but not in strains with alterations that elevate or reduce hot spot activity. The position and intensity of micrococcal nuclease-hypersensitive sites correlate poorly with the sites of DSB formation.
Full Text
Duke Authors
Cited Authors
- Fan, QQ; Petes, TD
Published Date
- May 1996
Published In
Volume / Issue
- 16 / 5
Start / End Page
- 2037 - 2043
PubMed ID
- 8628269
Pubmed Central ID
- PMC231190
International Standard Serial Number (ISSN)
- 0270-7306
Digital Object Identifier (DOI)
- 10.1128/MCB.16.5.2037
Language
- eng
Conference Location
- United States