Relationship between nuclease-hypersensitive sites and meiotic recombination hot spot activity at the HIS4 locus of Saccharomyces cerevisiae.

Journal Article (Journal Article)

Meiotic double-strand DNA breaks (DSBs), the lesions that initiate meiotic recombination at the HIS4 recombination hot spot, occur in a region upstream of the coding sequence associated with multiple DNase I-hypersensitive sites. Mutations in transcription factors that lead to loss of the DSBs result in the loss of some but not all DNase I-hypersensitive sites in the upstream region. A meiosis-specific change in chromatin structure is detected in strains with the wild-type hot spot but not in strains with alterations that elevate or reduce hot spot activity. The position and intensity of micrococcal nuclease-hypersensitive sites correlate poorly with the sites of DSB formation.

Full Text

Duke Authors

Cited Authors

  • Fan, QQ; Petes, TD

Published Date

  • May 1996

Published In

Volume / Issue

  • 16 / 5

Start / End Page

  • 2037 - 2043

PubMed ID

  • 8628269

Pubmed Central ID

  • PMC231190

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/MCB.16.5.2037


  • eng

Conference Location

  • United States