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Relationship between nuclease-hypersensitive sites and meiotic recombination hot spot activity at the HIS4 locus of Saccharomyces cerevisiae.

Publication ,  Journal Article
Fan, QQ; Petes, TD
Published in: Mol Cell Biol
May 1996

Meiotic double-strand DNA breaks (DSBs), the lesions that initiate meiotic recombination at the HIS4 recombination hot spot, occur in a region upstream of the coding sequence associated with multiple DNase I-hypersensitive sites. Mutations in transcription factors that lead to loss of the DSBs result in the loss of some but not all DNase I-hypersensitive sites in the upstream region. A meiosis-specific change in chromatin structure is detected in strains with the wild-type hot spot but not in strains with alterations that elevate or reduce hot spot activity. The position and intensity of micrococcal nuclease-hypersensitive sites correlate poorly with the sites of DSB formation.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

May 1996

Volume

16

Issue

5

Start / End Page

2037 / 2043

Location

United States

Related Subject Headings

  • Transcription Factors
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Restriction Mapping
  • Recombination, Genetic
  • Pyrophosphatases
  • Promoter Regions, Genetic
  • Multienzyme Complexes
  • Mitosis
  • Meiosis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Fan, Q. Q., & Petes, T. D. (1996). Relationship between nuclease-hypersensitive sites and meiotic recombination hot spot activity at the HIS4 locus of Saccharomyces cerevisiae. Mol Cell Biol, 16(5), 2037–2043. https://doi.org/10.1128/MCB.16.5.2037
Fan, Q. Q., and T. D. Petes. “Relationship between nuclease-hypersensitive sites and meiotic recombination hot spot activity at the HIS4 locus of Saccharomyces cerevisiae.Mol Cell Biol 16, no. 5 (May 1996): 2037–43. https://doi.org/10.1128/MCB.16.5.2037.
Fan, Q. Q., and T. D. Petes. “Relationship between nuclease-hypersensitive sites and meiotic recombination hot spot activity at the HIS4 locus of Saccharomyces cerevisiae.Mol Cell Biol, vol. 16, no. 5, May 1996, pp. 2037–43. Pubmed, doi:10.1128/MCB.16.5.2037.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

May 1996

Volume

16

Issue

5

Start / End Page

2037 / 2043

Location

United States

Related Subject Headings

  • Transcription Factors
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Restriction Mapping
  • Recombination, Genetic
  • Pyrophosphatases
  • Promoter Regions, Genetic
  • Multienzyme Complexes
  • Mitosis
  • Meiosis