Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast.
Journal Article (Journal Article)
Meiotic recombination in Saccharomyces cerevisiae is initiated by double-strand DNA breaks (DSBs). Using two approaches, we mapped the position of DSBs associated with a recombination hotspot created by insertion of telomeric sequences into the region upstream of HIS4. We found that the breaks have no obvious sequence specificity and localize to a region of approximately 50 bp adjacent to the telomeric insertion. By mapping the breaks and by studies of the exonuclease III sensitivity of the broken ends, we conclude that most of the broken DNA molecules have blunt ends with 3'-hydroxyl groups.
Full Text
Duke Authors
Cited Authors
- Xu, F; Petes, TD
Published Date
- July 1996
Published In
Volume / Issue
- 143 / 3
Start / End Page
- 1115 - 1125
PubMed ID
- 8807286
Pubmed Central ID
- PMC1207383
International Standard Serial Number (ISSN)
- 0016-6731
Digital Object Identifier (DOI)
- 10.1093/genetics/143.3.1115
Language
- eng
Conference Location
- United States