Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast.
Publication
, Journal Article
Xu, F; Petes, TD
Published in: Genetics
July 1996
Meiotic recombination in Saccharomyces cerevisiae is initiated by double-strand DNA breaks (DSBs). Using two approaches, we mapped the position of DSBs associated with a recombination hotspot created by insertion of telomeric sequences into the region upstream of HIS4. We found that the breaks have no obvious sequence specificity and localize to a region of approximately 50 bp adjacent to the telomeric insertion. By mapping the breaks and by studies of the exonuclease III sensitivity of the broken ends, we conclude that most of the broken DNA molecules have blunt ends with 3'-hydroxyl groups.
Duke Scholars
Published In
Genetics
DOI
ISSN
0016-6731
Publication Date
July 1996
Volume
143
Issue
3
Start / End Page
1115 / 1125
Location
United States
Related Subject Headings
- Transcription Factors
- Telomere
- Saccharomyces cerevisiae Proteins
- Saccharomyces cerevisiae
- Recombination, Genetic
- Pyrophosphatases
- Polymerase Chain Reaction
- Mutagenesis, Insertional
- Molecular Sequence Data
- Meiosis
Citation
APA
Chicago
ICMJE
MLA
NLM
Xu, F., & Petes, T. D. (1996). Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast. Genetics, 143(3), 1115–1125. https://doi.org/10.1093/genetics/143.3.1115
Xu, F., and T. D. Petes. “Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast.” Genetics 143, no. 3 (July 1996): 1115–25. https://doi.org/10.1093/genetics/143.3.1115.
Xu F, Petes TD. Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast. Genetics. 1996 Jul;143(3):1115–25.
Xu, F., and T. D. Petes. “Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast.” Genetics, vol. 143, no. 3, July 1996, pp. 1115–25. Pubmed, doi:10.1093/genetics/143.3.1115.
Xu F, Petes TD. Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast. Genetics. 1996 Jul;143(3):1115–1125.
Published In
Genetics
DOI
ISSN
0016-6731
Publication Date
July 1996
Volume
143
Issue
3
Start / End Page
1115 / 1125
Location
United States
Related Subject Headings
- Transcription Factors
- Telomere
- Saccharomyces cerevisiae Proteins
- Saccharomyces cerevisiae
- Recombination, Genetic
- Pyrophosphatases
- Polymerase Chain Reaction
- Mutagenesis, Insertional
- Molecular Sequence Data
- Meiosis