A novel structural form of the 2 micron plasmid of the yeast Saccharomyces cerevisiae.


Journal Article

DNA was isolated from cells of Saccharomyces cerevisiae incubated under conditions that enriched for DNA replication intermediates. A novel form of the 2 microns plasmid was detected, in which two monomeric or dimeric circles were joined by a linear double-stranded segment of variable length. We suggest that this molecule is a consequence of site-specific recombination within a dimeric DNA molecule during DNA replication. The existence of this molecule provides supporting physical evidence for a variant of the model of 2 mu plasmid amplification first proposed by Futcher (1986).

Full Text

Duke Authors

Cited Authors

  • Petes, TD; Williamson, DH

Published Date

  • October 1994

Published In

Volume / Issue

  • 10 / 10

Start / End Page

  • 1341 - 1345

PubMed ID

  • 7900423

Pubmed Central ID

  • 7900423

International Standard Serial Number (ISSN)

  • 0749-503X

Digital Object Identifier (DOI)

  • 10.1002/yea.320101011


  • eng

Conference Location

  • England