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Instability of a plasmid-borne inverted repeat in Saccharomyces cerevisiae.

Publication ,  Journal Article
Henderson, ST; Petes, TD
Published in: Genetics
May 1993

Inverted repeated DNA sequences are common in both prokaryotes and eukaryotes. We found that a plasmid-borne 94 base-pair inverted repeat (a perfect palindrome of 47 bp) containing a poly GT sequence is unstable in S. cerevisiae, with a minimal deletion frequency of about 10(-4)/mitotic division. Ten independent deletions had identical end points. Sequence analysis indicated that all deletions were the result of a DNA polymerase slippage event (or a recombination event) involving a 5-bp repeat (5' CGACG 3') that flanked the inverted repeat. The deletion rate and the types of deletions were unaffected by the rad52 mutation. Strains with the pms1 mutation had a 10-fold elevated frequency of instability of the inverted repeat. The types of sequence alterations observed in the pms1 background, however, were different than those seen in either the wild-type or rad52 genetic backgrounds.

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Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

May 1993

Volume

134

Issue

1

Start / End Page

57 / 62

Location

United States

Related Subject Headings

  • Sequence Deletion
  • Saccharomyces cerevisiae
  • Repetitive Sequences, Nucleic Acid
  • Nucleic Acid Conformation
  • Molecular Sequence Data
  • Models, Genetic
  • Developmental Biology
  • DNA, Fungal
  • DNA Repair
  • Base Sequence
 

Citation

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Henderson, S. T., & Petes, T. D. (1993). Instability of a plasmid-borne inverted repeat in Saccharomyces cerevisiae. Genetics, 134(1), 57–62. https://doi.org/10.1093/genetics/134.1.57
Henderson, S. T., and T. D. Petes. “Instability of a plasmid-borne inverted repeat in Saccharomyces cerevisiae.Genetics 134, no. 1 (May 1993): 57–62. https://doi.org/10.1093/genetics/134.1.57.
Henderson ST, Petes TD. Instability of a plasmid-borne inverted repeat in Saccharomyces cerevisiae. Genetics. 1993 May;134(1):57–62.
Henderson, S. T., and T. D. Petes. “Instability of a plasmid-borne inverted repeat in Saccharomyces cerevisiae.Genetics, vol. 134, no. 1, May 1993, pp. 57–62. Pubmed, doi:10.1093/genetics/134.1.57.
Henderson ST, Petes TD. Instability of a plasmid-borne inverted repeat in Saccharomyces cerevisiae. Genetics. 1993 May;134(1):57–62.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

May 1993

Volume

134

Issue

1

Start / End Page

57 / 62

Location

United States

Related Subject Headings

  • Sequence Deletion
  • Saccharomyces cerevisiae
  • Repetitive Sequences, Nucleic Acid
  • Nucleic Acid Conformation
  • Molecular Sequence Data
  • Models, Genetic
  • Developmental Biology
  • DNA, Fungal
  • DNA Repair
  • Base Sequence