Instability of a plasmid-borne inverted repeat in Saccharomyces cerevisiae.
Journal Article (Journal Article)
Inverted repeated DNA sequences are common in both prokaryotes and eukaryotes. We found that a plasmid-borne 94 base-pair inverted repeat (a perfect palindrome of 47 bp) containing a poly GT sequence is unstable in S. cerevisiae, with a minimal deletion frequency of about 10(-4)/mitotic division. Ten independent deletions had identical end points. Sequence analysis indicated that all deletions were the result of a DNA polymerase slippage event (or a recombination event) involving a 5-bp repeat (5' CGACG 3') that flanked the inverted repeat. The deletion rate and the types of deletions were unaffected by the rad52 mutation. Strains with the pms1 mutation had a 10-fold elevated frequency of instability of the inverted repeat. The types of sequence alterations observed in the pms1 background, however, were different than those seen in either the wild-type or rad52 genetic backgrounds.
Full Text
Duke Authors
Cited Authors
- Henderson, ST; Petes, TD
Published Date
- May 1993
Published In
Volume / Issue
- 134 / 1
Start / End Page
- 57 - 62
PubMed ID
- 8514149
Pubmed Central ID
- PMC1205444
International Standard Serial Number (ISSN)
- 0016-6731
Digital Object Identifier (DOI)
- 10.1093/genetics/134.1.57
Language
- eng
Conference Location
- United States