Instability of simple sequence DNA in Saccharomyces cerevisiae.

Journal Article

All eukaryotic genomes thus far examined contain simple sequence repeats. A particularly common simple sequence in many organisms (including humans) consists of tracts of alternating GT residues on one strand. Allelic poly(GT) tracts are often of different lengths in different individuals, indicating that they are likely to be unstable. We examined the instability of poly(GT) and poly(G) tracts in the yeast Saccharomyces cerevisiae. We found that these tracts were dramatically unstable, altering length at a minimal rate of 10(-4) events per division. Most of the changes involved one or two repeat unit additions or deletions, although one alteration involved an interaction with the yeast telomeres.

Full Text

Duke Authors

Cited Authors

  • Henderson, ST; Petes, TD

Published Date

  • June 1992

Published In

Volume / Issue

  • 12 / 6

Start / End Page

  • 2749 - 2757

PubMed ID

  • 1588966

International Standard Serial Number (ISSN)

  • 0270-7306

Language

  • eng

Conference Location

  • United States