Analysis of a gene conversion gradient at the HIS4 locus in Saccharomyces cerevisiae.

Journal Article (Journal Article)

Heteroduplexes formed between genes on homologous chromosomes are intermediates in meiotic recombination. In the HIS4 gene of Saccharomyces cerevisiae, most mutant alleles at the 5' end of the gene have a higher rate of meiotic recombination (gene conversion) than mutant alleles at the 3' end of the gene. Such gradients are usually interpreted as indicating a higher frequency of heteroduplex formation at the high conversion end of the gene. We present evidence indicating that the gradient of conversion at HIS4 primarily reflects the direction of mismatch repair rather than the frequency of heteroduplex formation. We also identify a site located between the 5' end of HIS4 and the 3' end of BIK1 that stimulates heteroduplex formation at HIS4 and BIK1.

Full Text

Duke Authors

Cited Authors

  • Detloff, P; White, MA; Petes, TD

Published Date

  • September 1992

Published In

Volume / Issue

  • 132 / 1

Start / End Page

  • 113 - 123

PubMed ID

  • 1398048

Pubmed Central ID

  • PMC1205110

International Standard Serial Number (ISSN)

  • 0016-6731

Digital Object Identifier (DOI)

  • 10.1093/genetics/132.1.113


  • eng

Conference Location

  • United States