Repair of specific base pair mismatches formed during meiotic recombination in the yeast Saccharomyces cerevisiae.

Journal Article (Journal Article)

Heteroduplexes formed between DNA strands derived from different homologous chromosomes are an intermediate in meiotic crossing over in the yeast Saccharomyces cerevisiae and other eucaryotes. A heteroduplex formed between wild-type and mutant genes will contain a base pair mismatch; failure to repair this mismatch will lead to postmeiotic segregation (PMS). By analyzing the frequency of PMS for various mutant alleles in the yeast HIS4 gene, we showed that C/C mismatches were inefficiently repaired relative to all other point mismatches. These other mismatches (G/G, G/A, T/T, A/A, T/C, C/A, A/A, and T/G) were repaired with approximately the same efficiency. We found that in spores with unrepaired mismatches in heteroduplexes, the nontranscribed strand of the HIS4 gene was more frequently donated than the transcribed strand. In addition, the direction of repair for certain mismatches was nonrandom.

Full Text

Duke Authors

Cited Authors

  • Detloff, P; Sieber, J; Petes, TD

Published Date

  • February 1991

Published In

Volume / Issue

  • 11 / 2

Start / End Page

  • 737 - 745

PubMed ID

  • 1990280

Pubmed Central ID

  • PMC359725

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.11.2.737-745.1991


  • eng

Conference Location

  • United States