Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae.

Journal Article (Journal Article)

DNA fragments (generated by BamHI treatment) with no homology to the yeast genome were transformed into Saccharomyces cerevisiae. When the fragments were transformed in the presence of the BamHI enzyme, they integrated into genomic BamHI sites. When the fragments were transformed in the absence of the enzyme, they integrated into genomic G-A-T-C sites. Since the G-A-T-C sequence is present at the ends of BamHI fragments, this results indicates that four base pairs of homology are sufficient for some types of mitotic recombination.

Full Text

Duke Authors

Cited Authors

  • Schiestl, RH; Petes, TD

Published Date

  • September 1, 1991

Published In

Volume / Issue

  • 88 / 17

Start / End Page

  • 7585 - 7589

PubMed ID

  • 1881899

Pubmed Central ID

  • 1881899

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.88.17.7585

Language

  • eng

Conference Location

  • United States