Palindromic sequences in heteroduplex DNA inhibit mismatch repair in yeast.

Published

Journal Article

Although single heterozygous markers in yeast usually segregate during meiosis in a 2:2 ratio, abberant 3:1 segregations occur quite frequently as a result of gene-conversion events. A second type of aberrant segregation, post-meiotic segregation, results from the segregation of two genotypes from a single haploid spore; in yeast such events are detected as sectored spore colonies and usually occur rarely. Post-meiotic segregation is thought to result from the replication of heteroduplex DNA formed during meiotic recombination. We report here that if the heteroduplex includes a palindromic insertion sequence, a high frequency of post-meiotic segregation results. This suggests that palindromic insertions are poorly repaired, which may be the result of hairpin-loop formation that affects the efficiency of repair of heteroduplex DNA.

Full Text

Duke Authors

Cited Authors

  • Nag, DK; White, MA; Petes, TD

Published Date

  • July 27, 1989

Published In

Volume / Issue

  • 340 / 6231

Start / End Page

  • 318 - 320

PubMed ID

  • 2546083

Pubmed Central ID

  • 2546083

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/340318a0

Language

  • eng

Conference Location

  • England