Palindromic sequences in heteroduplex DNA inhibit mismatch repair in yeast.
Journal Article (Journal Article)
Although single heterozygous markers in yeast usually segregate during meiosis in a 2:2 ratio, abberant 3:1 segregations occur quite frequently as a result of gene-conversion events. A second type of aberrant segregation, post-meiotic segregation, results from the segregation of two genotypes from a single haploid spore; in yeast such events are detected as sectored spore colonies and usually occur rarely. Post-meiotic segregation is thought to result from the replication of heteroduplex DNA formed during meiotic recombination. We report here that if the heteroduplex includes a palindromic insertion sequence, a high frequency of post-meiotic segregation results. This suggests that palindromic insertions are poorly repaired, which may be the result of hairpin-loop formation that affects the efficiency of repair of heteroduplex DNA.
Full Text
Duke Authors
Cited Authors
- Nag, DK; White, MA; Petes, TD
Published Date
- July 27, 1989
Published In
Volume / Issue
- 340 / 6231
Start / End Page
- 318 - 320
PubMed ID
- 2546083
International Standard Serial Number (ISSN)
- 0028-0836
Digital Object Identifier (DOI)
- 10.1038/340318a0
Language
- eng
Conference Location
- England