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Isolation and characterization of a Ty element inserted into the ribosomal DNA of the yeast Saccharomyces cerevisiae.

Publication ,  Journal Article
Vincent, A; Petes, TD
Published in: Nucleic Acids Res
April 11, 1986

The yeast Saccharomyces cerevisiae has about 30 to 50 copies of a transposable element Ty. Most of these elements are located at the 5' ends of protein coding sequences and are flanked by a 5 bp duplication. We report below an insertion of a Ty element into one of the repeated ribosomal RNA (rRNA) genes of yeast. The element is located between the 3' ends of the divergentally transcribed 37S and 5S rRNA's and is not flanked by a 5 bp duplication. In addition, one end of the Ty insertion is contiguous with a 306 bp deletion of the sequences of the rRNA gene. We find that this insertion, unlike most Ty insertions, is mitotically unstable.

Duke Scholars

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

April 11, 1986

Volume

14

Issue

7

Start / End Page

2939 / 2949

Location

England

Related Subject Headings

  • Saccharomyces cerevisiae
  • Repetitive Sequences, Nucleic Acid
  • RNA, Ribosomal
  • Developmental Biology
  • DNA, Ribosomal
  • DNA Transposable Elements
  • DNA Restriction Enzymes
  • Base Sequence
  • 41 Environmental sciences
  • 34 Chemical sciences
 

Citation

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Vincent, A., & Petes, T. D. (1986). Isolation and characterization of a Ty element inserted into the ribosomal DNA of the yeast Saccharomyces cerevisiae. Nucleic Acids Res, 14(7), 2939–2949. https://doi.org/10.1093/nar/14.7.2939
Vincent, A., and T. D. Petes. “Isolation and characterization of a Ty element inserted into the ribosomal DNA of the yeast Saccharomyces cerevisiae.Nucleic Acids Res 14, no. 7 (April 11, 1986): 2939–49. https://doi.org/10.1093/nar/14.7.2939.
Vincent, A., and T. D. Petes. “Isolation and characterization of a Ty element inserted into the ribosomal DNA of the yeast Saccharomyces cerevisiae.Nucleic Acids Res, vol. 14, no. 7, Apr. 1986, pp. 2939–49. Pubmed, doi:10.1093/nar/14.7.2939.
Journal cover image

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

April 11, 1986

Volume

14

Issue

7

Start / End Page

2939 / 2949

Location

England

Related Subject Headings

  • Saccharomyces cerevisiae
  • Repetitive Sequences, Nucleic Acid
  • RNA, Ribosomal
  • Developmental Biology
  • DNA, Ribosomal
  • DNA Transposable Elements
  • DNA Restriction Enzymes
  • Base Sequence
  • 41 Environmental sciences
  • 34 Chemical sciences