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Production of hydroxyl radical in the hippocampus after CO hypoxia or hypoxic hypoxia in the rat.

Publication ,  Journal Article
Piantadosi, CA; Zhang, J; Demchenko, IT
Published in: Free Radic Biol Med
1997

Carbon monoxide poisoning produces both immediate and delayed neuronal injury in selective regions of the brain that is not readily explained on the basis of tissue hypoxia. One possibility is that cellular injury during and after CO poisoning is related to the production of reactive oxygen species (ROS) by the brain. In this study, we hypothesized that the extent of ROS generation in the brain would be greater after CO than after hypoxic hypoxia due to intracellular uptake of CO. We assessed hydroxyl radical (OH.) production by comparing the nonenzymatic hydroxylation of salicylic acid to 2,3-dihydroxybenzoic acid (2,3-DHBA) in the hippocampus of the rat by microdialysis during either CO hypoxia or an exposure to hypoxic hypoxia that produced similar PO2 and cerebral blood flow (CBF) values in the region of microdialysis. We found neither control animals nor animals exposed to 30 min of hypoxic hypoxia at a mean tissue PO2 of 15 mmHg demonstrated significant increases in 2,3-DHBA production in the hippocampus over the 2-h the exposure. In contrast, CO exposed rats which also developed brain PO2 values in the range of 15 mmHg showed highly significant increases in 2,3-DHBA production. We conclude that cerebral oxidative stress in the hippocampus of the rat during CO hypoxia in vivo is not a direct effect of decreased tissue oxygen concentration.

Duke Scholars

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Published In

Free Radic Biol Med

DOI

ISSN

0891-5849

Publication Date

1997

Volume

22

Issue

4

Start / End Page

725 / 732

Location

United States

Related Subject Headings

  • Salicylic Acid
  • Salicylates
  • Reactive Oxygen Species
  • Rats, Sprague-Dawley
  • Rats
  • Oxygen Consumption
  • Oxidative Stress
  • Male
  • Hypoxia
  • Hydroxylation
 

Citation

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Piantadosi, C. A., Zhang, J., & Demchenko, I. T. (1997). Production of hydroxyl radical in the hippocampus after CO hypoxia or hypoxic hypoxia in the rat. Free Radic Biol Med, 22(4), 725–732. https://doi.org/10.1016/s0891-5849(96)00423-6
Piantadosi, C. A., J. Zhang, and I. T. Demchenko. “Production of hydroxyl radical in the hippocampus after CO hypoxia or hypoxic hypoxia in the rat.Free Radic Biol Med 22, no. 4 (1997): 725–32. https://doi.org/10.1016/s0891-5849(96)00423-6.
Piantadosi CA, Zhang J, Demchenko IT. Production of hydroxyl radical in the hippocampus after CO hypoxia or hypoxic hypoxia in the rat. Free Radic Biol Med. 1997;22(4):725–32.
Piantadosi, C. A., et al. “Production of hydroxyl radical in the hippocampus after CO hypoxia or hypoxic hypoxia in the rat.Free Radic Biol Med, vol. 22, no. 4, 1997, pp. 725–32. Pubmed, doi:10.1016/s0891-5849(96)00423-6.
Piantadosi CA, Zhang J, Demchenko IT. Production of hydroxyl radical in the hippocampus after CO hypoxia or hypoxic hypoxia in the rat. Free Radic Biol Med. 1997;22(4):725–732.
Journal cover image

Published In

Free Radic Biol Med

DOI

ISSN

0891-5849

Publication Date

1997

Volume

22

Issue

4

Start / End Page

725 / 732

Location

United States

Related Subject Headings

  • Salicylic Acid
  • Salicylates
  • Reactive Oxygen Species
  • Rats, Sprague-Dawley
  • Rats
  • Oxygen Consumption
  • Oxidative Stress
  • Male
  • Hypoxia
  • Hydroxylation