Production of prostaglandin E₂ in response to infection with modified vaccinia Ankara virus.

Published

Journal Article

Prostaglandin E₂ (PGE₂) is an arachidonic acid (AA)-derived signaling molecule that can influence host immune responses to infection or vaccination. In this study, we investigated PGE₂ production in vitro by cells infected with the poxvirus vaccine strain, modified vaccinia Ankara virus (MVA). Human THP-1 cells, murine bone marrow-derived dendritic cells, and murine C3HA fibroblasts all accumulated PGE₂ to high levels in culture supernatants upon infection with MVA. We also demonstrated that MVA induced the release of AA from infected cells, and this was, most unusually, independent of host cytosolic phospholipase A₂ activity. The accumulation of AA and PGE₂ was dependent on viral gene expression, but independent of canonical NF-κB signaling via p65/RelA. The production of PGE₂ required host cyclooxygenase-2 (COX-2) activity, and COX-2 protein accumulated during MVA infection. The results of this study provide insight into a novel aspect of MVA biology that may affect the efficacy of MVA-based vaccines.

Full Text

Duke Authors

Cited Authors

  • Pollara, JJ; Spesock, AH; Pickup, DJ; Laster, SM; Petty, ITD

Published Date

  • July 5, 2012

Published In

Volume / Issue

  • 428 / 2

Start / End Page

  • 146 - 155

PubMed ID

  • 22534090

Pubmed Central ID

  • 22534090

Electronic International Standard Serial Number (EISSN)

  • 1096-0341

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2012.03.019

Language

  • eng

Conference Location

  • United States