Production of prostaglandin E₂ in response to infection with modified vaccinia Ankara virus.
Journal Article (Journal Article)
Prostaglandin E₂ (PGE₂) is an arachidonic acid (AA)-derived signaling molecule that can influence host immune responses to infection or vaccination. In this study, we investigated PGE₂ production in vitro by cells infected with the poxvirus vaccine strain, modified vaccinia Ankara virus (MVA). Human THP-1 cells, murine bone marrow-derived dendritic cells, and murine C3HA fibroblasts all accumulated PGE₂ to high levels in culture supernatants upon infection with MVA. We also demonstrated that MVA induced the release of AA from infected cells, and this was, most unusually, independent of host cytosolic phospholipase A₂ activity. The accumulation of AA and PGE₂ was dependent on viral gene expression, but independent of canonical NF-κB signaling via p65/RelA. The production of PGE₂ required host cyclooxygenase-2 (COX-2) activity, and COX-2 protein accumulated during MVA infection. The results of this study provide insight into a novel aspect of MVA biology that may affect the efficacy of MVA-based vaccines.
Full Text
Duke Authors
Cited Authors
- Pollara, JJ; Spesock, AH; Pickup, DJ; Laster, SM; Petty, ITD
Published Date
- July 5, 2012
Published In
Volume / Issue
- 428 / 2
Start / End Page
- 146 - 155
PubMed ID
- 22534090
Pubmed Central ID
- PMC3353002
Electronic International Standard Serial Number (EISSN)
- 1096-0341
Digital Object Identifier (DOI)
- 10.1016/j.virol.2012.03.019
Language
- eng
Conference Location
- United States