Regeneration of amputated zebrafish fin rays from de novo osteoblasts.

Published

Journal Article

Determining the cellular source of new skeletal elements is critical for understanding appendage regeneration in amphibians and fish. Recent lineage-tracing studies indicated that zebrafish fin ray bone regenerates through the dedifferentiation and proliferation of spared osteoblasts, with limited if any contribution from other cell types. Here, we examined the requirement for this mechanism by using genetic ablation techniques to destroy virtually all skeletal osteoblasts in adult zebrafish fins. Animals survived this injury and restored the osteoblast population within 2 weeks. Moreover, amputated fins depleted of osteoblasts regenerated new fin ray structures at rates indistinguishable from fins possessing a resident osteoblast population. Inducible genetic fate mapping confirmed that new bone cells do not arise from dedifferentiated osteoblasts under these conditions. Our findings demonstrate diversity in the cellular origins of appendage bone and reveal that de novo osteoblasts can fully support the regeneration of amputated zebrafish fins.

Full Text

Duke Authors

Cited Authors

  • Singh, SP; Holdway, JE; Poss, KD

Published Date

  • April 17, 2012

Published In

Volume / Issue

  • 22 / 4

Start / End Page

  • 879 - 886

PubMed ID

  • 22516203

Pubmed Central ID

  • 22516203

Electronic International Standard Serial Number (EISSN)

  • 1878-1551

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2012.03.006

Language

  • eng

Conference Location

  • United States