Modulation of ATP-induced calcium signaling by progesterone in T47D-Y breast cancer cells.

Journal Article (Journal Article)

Extracellular ATP activates purinergic (P(2)) receptors with an increase in intracellular calcium and phosphorylation of MAPK. In this study we have investigated the effect of progesterone/progestin on ATP-induced calcium mobilization and phosphorylation of the kinase ERK in the T47D-Y breast cancer cell line that exhibits no detectable nuclear progesterone receptor expression. Brief pretreatment with progesterone/progestin results in a dose dependent inhibition of ATP-induced intracellular calcium mobilization, and inhibition of ERK phosphorylation. Response to a cell impermeable ligand and inhibition of the response by an inactivating antibody suggests a mechanism of action at the plasma membrane. These results in T47D-Y cells strongly suggest that progesterone can act in a rapid non-nuclear manner to inhibit extracellular ATP effects on intracellular calcium mobilization and ERK activation. This research provides an example of progesterone action in a breast cancer cell line lacking expression of the classical nuclear progesterone receptors.

Full Text

Duke Authors

Cited Authors

  • Lee, KL; Dai, Q; Hansen, EL; Saner, CN; Price, TM

Published Date

  • May 5, 2010

Published In

Volume / Issue

  • 319 / 1-2

Start / End Page

  • 109 - 115

PubMed ID

  • 20079401

Pubmed Central ID

  • PMC2837125

Electronic International Standard Serial Number (EISSN)

  • 1872-8057

Digital Object Identifier (DOI)

  • 10.1016/j.mce.2010.01.004


  • eng

Conference Location

  • Ireland