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Transmission electron (TEM) and Light Microscopic (LM) analysis of hyperopic and myopic corrections induced by a photosensitizing dye and the argon laser in a rabbit model

Publication ,  Journal Article
Rose, JG; Fowler, WC; Proia, AD; Iacobucci, MJ; Vietorisz, EC; Straub, KD; Ross, JR; Barletta, JP; Plunkett, MA
Published in: Investigative Ophthalmology and Visual Science
February 15, 1996

Purpose: To study the histopathologic effects and possible collagen fiber alterations in rabbits previously shown to exhibit refractive change by treatment with a photoactivated chromophore and the argon laser while maintaining clear corneas. Methods: Two New Zealand white rabbits at least 4 months postoperative were selected for analysis. One rabbit had undergone 4D hyperopic refractive treatment which regressed, while the other maintained 2.3D of myopic refractive change. The rabbits were anesthetized, and corneas excised prior to euthanasia. The treated and untreated corneas of each rabbit were promptly bisected with a cryostat blade through the treatment zone, and half of each cornea immediately fixed in 2% glutaraldehyde for TEM and the other half in a formaldehyde/glutaraldehyde mixture for LM stains (H&E, Masson-trichrome, colloidal iron). Results: TEM on treated and untreated eyes showed no obvious differences when examined by a masked corneal pathologist. Masked LM analysis disclosed only a slightly thicker acellular zone beneath the epithelium with a very subtle increase in collagen irregularity in the myopic treated cornea. TEM findings from deepithelialized, dye-treated control corneas are in progress. Conclusions: 1. TEM and LM analysis of refractive corrections induced by a photosensitizing dye and the argon laser did not reveal evidence of corneal denaturation or obvious differences to explain the refractive changes. 2. Although not identified on these histopathologic specimens, collagen crosslinking is speculated to be the responsible keratorefractive mechanism; further studies on this possible mechanism are in progress.

Duke Scholars

Published In

Investigative Ophthalmology and Visual Science

ISSN

0146-0404

Publication Date

February 15, 1996

Volume

37

Issue

3

Related Subject Headings

  • Ophthalmology & Optometry
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
 

Citation

APA
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MLA
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Rose, J. G., Fowler, W. C., Proia, A. D., Iacobucci, M. J., Vietorisz, E. C., Straub, K. D., … Plunkett, M. A. (1996). Transmission electron (TEM) and Light Microscopic (LM) analysis of hyperopic and myopic corrections induced by a photosensitizing dye and the argon laser in a rabbit model. Investigative Ophthalmology and Visual Science, 37(3).
Rose, J. G., W. C. Fowler, A. D. Proia, M. J. Iacobucci, E. C. Vietorisz, K. D. Straub, J. R. Ross, J. P. Barletta, and M. A. Plunkett. “Transmission electron (TEM) and Light Microscopic (LM) analysis of hyperopic and myopic corrections induced by a photosensitizing dye and the argon laser in a rabbit model.” Investigative Ophthalmology and Visual Science 37, no. 3 (February 15, 1996).
Rose JG, Fowler WC, Proia AD, Iacobucci MJ, Vietorisz EC, Straub KD, et al. Transmission electron (TEM) and Light Microscopic (LM) analysis of hyperopic and myopic corrections induced by a photosensitizing dye and the argon laser in a rabbit model. Investigative Ophthalmology and Visual Science. 1996 Feb 15;37(3).
Rose JG, Fowler WC, Proia AD, Iacobucci MJ, Vietorisz EC, Straub KD, Ross JR, Barletta JP, Plunkett MA. Transmission electron (TEM) and Light Microscopic (LM) analysis of hyperopic and myopic corrections induced by a photosensitizing dye and the argon laser in a rabbit model. Investigative Ophthalmology and Visual Science. 1996 Feb 15;37(3).

Published In

Investigative Ophthalmology and Visual Science

ISSN

0146-0404

Publication Date

February 15, 1996

Volume

37

Issue

3

Related Subject Headings

  • Ophthalmology & Optometry
  • 11 Medical and Health Sciences
  • 06 Biological Sciences