Novel circular DNA viruses in stool samples of wild-living chimpanzees.

Published

Journal Article

Viral particles in stool samples from wild-living chimpanzees were analysed using random PCR amplification and sequencing. Sequences encoding proteins distantly related to the replicase protein of single-stranded circular DNA viruses were identified. Inverse PCR was used to amplify and sequence multiple small circular DNA viral genomes. The viral genomes were related in size and genome organization to vertebrate circoviruses and plant geminiviruses but with a different location for the stem-loop structure involved in rolling circle DNA replication. The replicase genes of these viruses were most closely related to those of the much smaller (approximately 1 kb) plant nanovirus circular DNA chromosomes. Because the viruses have characteristics of both animal and plant viruses, we named them chimpanzee stool-associated circular viruses (ChiSCV). Further metagenomic studies of animal samples will greatly increase our knowledge of viral diversity and evolution.

Full Text

Duke Authors

Cited Authors

  • Blinkova, O; Victoria, J; Li, Y; Keele, BF; Sanz, C; Ndjango, J-BN; Peeters, M; Travis, D; Lonsdorf, EV; Wilson, ML; Pusey, AE; Hahn, BH; Delwart, EL

Published Date

  • January 2010

Published In

Volume / Issue

  • 91 / Pt 1

Start / End Page

  • 74 - 86

PubMed ID

  • 19759238

Pubmed Central ID

  • 19759238

Electronic International Standard Serial Number (EISSN)

  • 1465-2099

International Standard Serial Number (ISSN)

  • 0022-1317

Digital Object Identifier (DOI)

  • 10.1099/vir.0.015446-0

Language

  • eng