Induction of neutralizing antibodies and cytotoxic T lymphocytes in Balb/c mice immunized with virus-like particles presenting a gp120 molecule from a HIV-1 isolate of clade A.
Journal Article (Journal Article)
We have recently developed a candidate HIV-1 vaccine based on virus-like particles (VLPs) expressing a gp120 from an Ugandan HIV-1 isolate of the clade A (HIV-VLP(A)s). In vivo immunogenicity experiments were performed in Balb/c mice, with an immunization schedule based on a multiple-dose regimen of HIV-VLP(A)s without adjuvants, showing a significant induction of both humoral and cellular immunity. The Env-specific cellular response was investigated in vitro, scoring for both the proliferative response of T helper cells and the cytolytic activity of cytotoxic T lymphocytes (CTLs). Furthermore, immune sera showed >50% neutralization activity against both the autologous field isolate and the heterologous T cell adapted B-clade HIV-1(IIIB) viral strain. This is one of the first examples of HIV-1 vaccines based on antigens derived from the A clade, which represents >25% of all isolates identified world wide. In particular, the A clade is predominant in sub-Saharan countries, where 70% of the global HIV-1 infections occur, and where vaccination is the only rational strategy for an affordable prevention against HIV-1 infection.
Full Text
Duke Authors
Cited Authors
- Buonaguro, L; Racioppi, L; Tornesello, ML; Arra, C; Visciano, ML; Biryahwaho, B; Sempala, SDK; Giraldo, G; Buonaguro, FM
Published Date
- June 2002
Published In
Volume / Issue
- 54 / 3
Start / End Page
- 189 - 201
PubMed ID
- 12062391
International Standard Serial Number (ISSN)
- 0166-3542
Digital Object Identifier (DOI)
- 10.1016/s0166-3542(02)00004-9
Language
- eng
Conference Location
- Netherlands