Resveratrol arrests the cell division cycle at S/G2 phase transition.

Published

Journal Article

Resveratrol (3,5,4'-trihydroxystilbene) is a naturally occurring phytoalexin, found in grapes and wine, which has been reported to exert a variety of important pharmacological effects. We have investigated the activity of resveratrol on proliferation and differentiation of the promyelocitic cell line HL-60. A concentration as low as 30 microM causes a complete arrest of proliferation and a rapid induction of differentiation towards a myelo-monocytic phenotype. Analyses by flow cytometry showed the absence of the G2/M peak and the accumulation of cells in G1 and S phases. Moreover, at the concentrations employed, a very low amount of apoptotic cells was evidenced. A detailed biochemical analysis demonstrated that the G1 phase of the cell division cycle engine was completely unmodified by resveratrol addition, thus indicating that the G1 --> S transition occurs normally. Conversely, after only 24 h treatment, a significant increase of cyclins A and E could be observed along with the accumulation of cdc2 in the inactive phosphorylated form. These data demonstrate that resveratrol causes a complete and reversible cell cycle arrest at the S phase checkpoint.

Full Text

Duke Authors

Cited Authors

  • Ragione, FD; Cucciolla, V; Borriello, A; Pietra, VD; Racioppi, L; Soldati, G; Manna, C; Galletti, P; Zappia, V

Published Date

  • September 1998

Published In

Volume / Issue

  • 250 / 1

Start / End Page

  • 53 - 58

PubMed ID

  • 9735330

Pubmed Central ID

  • 9735330

Electronic International Standard Serial Number (EISSN)

  • 1090-2104

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1006/bbrc.1998.9263

Language

  • eng