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Resveratrol arrests the cell division cycle at S/G2 phase transition.

Publication ,  Journal Article
Ragione, FD; Cucciolla, V; Borriello, A; Pietra, VD; Racioppi, L; Soldati, G; Manna, C; Galletti, P; Zappia, V
Published in: Biochem Biophys Res Commun
September 8, 1998

Resveratrol (3,5,4'-trihydroxystilbene) is a naturally occurring phytoalexin, found in grapes and wine, which has been reported to exert a variety of important pharmacological effects. We have investigated the activity of resveratrol on proliferation and differentiation of the promyelocitic cell line HL-60. A concentration as low as 30 microM causes a complete arrest of proliferation and a rapid induction of differentiation towards a myelo-monocytic phenotype. Analyses by flow cytometry showed the absence of the G2/M peak and the accumulation of cells in G1 and S phases. Moreover, at the concentrations employed, a very low amount of apoptotic cells was evidenced. A detailed biochemical analysis demonstrated that the G1 phase of the cell division cycle engine was completely unmodified by resveratrol addition, thus indicating that the G1 --> S transition occurs normally. Conversely, after only 24 h treatment, a significant increase of cyclins A and E could be observed along with the accumulation of cdc2 in the inactive phosphorylated form. These data demonstrate that resveratrol causes a complete and reversible cell cycle arrest at the S phase checkpoint.

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Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

September 8, 1998

Volume

250

Issue

1

Start / End Page

53 / 58

Location

United States

Related Subject Headings

  • Stilbenes
  • S Phase
  • Resveratrol
  • Humans
  • HL-60 Cells
  • G2 Phase
  • DNA Replication
  • Cell Division
  • Cell Differentiation
  • Biochemistry & Molecular Biology
 

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Ragione, F. D., Cucciolla, V., Borriello, A., Pietra, V. D., Racioppi, L., Soldati, G., … Zappia, V. (1998). Resveratrol arrests the cell division cycle at S/G2 phase transition. Biochem Biophys Res Commun, 250(1), 53–58. https://doi.org/10.1006/bbrc.1998.9263
Ragione, F. D., V. Cucciolla, A. Borriello, V. D. Pietra, L. Racioppi, G. Soldati, C. Manna, P. Galletti, and V. Zappia. “Resveratrol arrests the cell division cycle at S/G2 phase transition.Biochem Biophys Res Commun 250, no. 1 (September 8, 1998): 53–58. https://doi.org/10.1006/bbrc.1998.9263.
Ragione FD, Cucciolla V, Borriello A, Pietra VD, Racioppi L, Soldati G, et al. Resveratrol arrests the cell division cycle at S/G2 phase transition. Biochem Biophys Res Commun. 1998 Sep 8;250(1):53–8.
Ragione, F. D., et al. “Resveratrol arrests the cell division cycle at S/G2 phase transition.Biochem Biophys Res Commun, vol. 250, no. 1, Sept. 1998, pp. 53–58. Pubmed, doi:10.1006/bbrc.1998.9263.
Ragione FD, Cucciolla V, Borriello A, Pietra VD, Racioppi L, Soldati G, Manna C, Galletti P, Zappia V. Resveratrol arrests the cell division cycle at S/G2 phase transition. Biochem Biophys Res Commun. 1998 Sep 8;250(1):53–58.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

September 8, 1998

Volume

250

Issue

1

Start / End Page

53 / 58

Location

United States

Related Subject Headings

  • Stilbenes
  • S Phase
  • Resveratrol
  • Humans
  • HL-60 Cells
  • G2 Phase
  • DNA Replication
  • Cell Division
  • Cell Differentiation
  • Biochemistry & Molecular Biology