Serum withdrawal induces apoptotic cell death in Ki-ras transformed but not in normal differentiated thyroid cells.
Journal Article (Journal Article)
Thyroid cells transformed by the Kirsten-ras oncogene become tumorigenic in syngeneic animals. Their growth is no longer dependent on TSH but becomes dependent on serum. Combining morphological and biochemical evidence, we show that serum withdrawal induces apoptotic cell death in Kirsten and Harvey-ras transformed thyroid cell. On the other hand, neither serum nor TSH withdrawal induce apoptosis in differentiated FRTL-5 cells. The induction of apoptosis by serum withdrawal is rapid and not triggered at a specific phase of the cell cycle. We suggest that induction of apoptosis following growth factor deprivation is an additional important characteristic, besides TSH-independence for growth and dedifferentiation, of the thyroid transformed phenotype.
Full Text
Duke Authors
Cited Authors
- di Jeso, B; Ulianich, L; Racioppi, L; D'Armiento, F; Feliciello, A; Pacifico, F; Consiglio, E; Formisano, S
Published Date
- September 25, 1995
Published In
Volume / Issue
- 214 / 3
Start / End Page
- 819 - 824
PubMed ID
- 7575550
International Standard Serial Number (ISSN)
- 0006-291X
Digital Object Identifier (DOI)
- 10.1006/bbrc.1995.2360
Language
- eng
Conference Location
- United States