Cell surface expression of major histocompatibility class I antigens is modulated by P-glycoprotein transporter.
Journal Article (Journal Article)
P-glycoprotein (Mdr1), a member of the ABC superfamily, is a pump able to transport several compounds across plasma membranes. It displays a high level of similarity with the MHC-linked transporters TAP1 and TAP2 which are involved in the delivery of immunogenic peptides across the endoplasmic reticulum. In the present study we analyze the P-glycoprotein's ability to interfere with the biosynthetic pathway of the MHC class I molecules. Our results show that P-glycoprotein is involved in the modulation of the MHC class I expression in multidrug-resistant tumor cell lines, COS1 cells transfected with mdr1 gene, and human T lymphocytes. Epitope screening evokes the possibility that P-glycoprotein induces a modulation of the different MHC class I forms expressed on the cell surface. We propose that P-glycoprotein is involved in the transport of antigenic protein fragments from the cytosol into the endoplasmic reticulum. The suggested mechanism could be physiologically relevant in tissues displaying a high Mdr1 activity, where this transporter could contribute to the regulation of locoregional immune responses.
Full Text
Duke Authors
Cited Authors
- Masci, AM; Scala, S; Racioppi, L; Zappacosta, S
Published Date
- March 1995
Published In
Volume / Issue
- 42 / 3
Start / End Page
- 245 - 253
PubMed ID
- 7759313
International Standard Serial Number (ISSN)
- 0198-8859
Digital Object Identifier (DOI)
- 10.1016/0198-8859(94)00096-9
Language
- eng
Conference Location
- United States