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Calibration schemes of a field-compatible optical spectroscopic system to quantify neovascular changes in the dysplastic cervix

Publication ,  Journal Article
Chang, VTC; Merisier, D; Yu, B; Walmer, DK; Ramanujam, N
Published in: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
May 2, 2011

A significant challenge in detecting cervical pre-cancer in low-resource settings is the lack of effective screening facilities and trained personnel to detect the disease before it is advanced. Light based technologies, particularly quantitative optical spectroscopy, have the potential to provide an effective, low cost, and portable solution for cervical pre-cancer screening in these communities. We have developed and characterized a portable USB-powered optical spectroscopic system to quantify total hemoglobin content, hemoglobin saturation, and reduced scattering coefficient of cervical tissue in vivo. The system consists of a high-power LED as light source, a bifurcated fiber optic assembly, and two USB spectrometers for sample and calibration spectra acquisitions. The system was subsequently tested in Leogane, Haiti, where diffuse reflectance spectra from 33 colposcopically normal sites in 21 patients were acquired. Two different calibration methods, i.e., a post-study diffuse reflectance standard measurement and a real time self-calibration channel were studied. Our results suggest that a self-calibration channel enabled more accurate extraction of scattering contrast through simultaneous real-time correction of intensity drifts in the system. A self-calibration system also minimizes operator bias and required training. Hence, future contact spectroscopy or imaging systems should incorporate a selfcalibration channel to reliably extract scattering contrast. © 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).

Duke Scholars

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

Publication Date

May 2, 2011

Volume

7891
 

Citation

APA
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Chang, V. T. C., Merisier, D., Yu, B., Walmer, D. K., & Ramanujam, N. (2011). Calibration schemes of a field-compatible optical spectroscopic system to quantify neovascular changes in the dysplastic cervix. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 7891. https://doi.org/10.1117/12.875855
Chang, V. T. C., D. Merisier, B. Yu, D. K. Walmer, and N. Ramanujam. “Calibration schemes of a field-compatible optical spectroscopic system to quantify neovascular changes in the dysplastic cervix.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE 7891 (May 2, 2011). https://doi.org/10.1117/12.875855.
Chang VTC, Merisier D, Yu B, Walmer DK, Ramanujam N. Calibration schemes of a field-compatible optical spectroscopic system to quantify neovascular changes in the dysplastic cervix. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2011 May 2;7891.
Chang, V. T. C., et al. “Calibration schemes of a field-compatible optical spectroscopic system to quantify neovascular changes in the dysplastic cervix.” Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7891, May 2011. Scopus, doi:10.1117/12.875855.
Chang VTC, Merisier D, Yu B, Walmer DK, Ramanujam N. Calibration schemes of a field-compatible optical spectroscopic system to quantify neovascular changes in the dysplastic cervix. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2011 May 2;7891.

Published In

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

DOI

ISSN

1605-7422

Publication Date

May 2, 2011

Volume

7891