Multiphoton redox ratio imaging for metabolic monitoring in vivo.


Journal Article

Metabolic monitoring at the cellular level in live tissues is important for understanding cell function, disease processes, and potential therapies. Multiphoton imaging of the relative amounts of NADH and FAD (the primary electron donor and acceptor, respectively, in the electron transport chain) provides a noninvasive method for monitoring cellular metabolic activity with high resolution in three dimensions in vivo. NADH and FAD are endogenous tissue fluorophores, and thus this method does not require exogenous stains or tissue excision. We describe the principles and protocols of multiphoton redox ratio imaging in vivo.

Full Text

Cited Authors

  • Skala, M; Ramanujam, N

Published Date

  • 2010

Published In

Volume / Issue

  • 594 /

Start / End Page

  • 155 - 162

PubMed ID

  • 20072916

Pubmed Central ID

  • 20072916

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-60761-411-1_11


  • eng

Conference Location

  • United States