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Selective expression of the small GTPase RhoB in the early developing mouse lens.

Publication ,  Journal Article
Maddala, R; Peng, YW; Rao, PV
Published in: Dev Dyn
November 2001

This report describes the expression and distribution pattern of RhoB GTPase in the developing mouse lens. RhoB expression was confirmed by sequencing an reverse transcriptase-polymerase chain reaction-generated DNA fragment of RhoB. Immunohistochemical analysis of RhoB revealed expression in the lens vesicle (both anterior and posterior vesicle) at embryonic day (E) 11.5, and in the epithelium and primary fibers of the E14.5 lens. Compared with the neonatal stage (day 1), where RhoB is detected in the entire lens (epithelium, primary, and secondary fibers), expression of this protein is restricted to the epithelial and outer cortical secondary fibers in postnatal lenses (from day 7 to day18). Interestingly, in E11.5 and E14.5 lenses, RhoB is localized predominantly in the lens, but not detectable in the retina, cornea, or other ocular tissues. RhoB expression appears to be down-regulated in the postnatal lens with concomitant up-regulation in the retina and cornea, compared with earlier stages of development (eyes of E11.5, E14.5, and neonatal mice). This study reveals the selective expression of RhoB in the lens during early eye development and suggests a potential role for this small GTPase in cytoskeletal reorganization associated with lens epithelial cell elongation and differentiation.

Duke Scholars

Published In

Dev Dyn

DOI

ISSN

1058-8388

Publication Date

November 2001

Volume

222

Issue

3

Start / End Page

534 / 537

Location

United States

Related Subject Headings

  • rhoB GTP-Binding Protein
  • Tissue Distribution
  • Reverse Transcriptase Polymerase Chain Reaction
  • Mice
  • Lens, Crystalline
  • In Vitro Techniques
  • Immunohistochemistry
  • Embryo, Mammalian
  • Developmental Biology
  • Blotting, Western
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Maddala, R., Peng, Y. W., & Rao, P. V. (2001). Selective expression of the small GTPase RhoB in the early developing mouse lens. Dev Dyn, 222(3), 534–537. https://doi.org/10.1002/dvdy.1202
Maddala, R., Y. W. Peng, and P. V. Rao. “Selective expression of the small GTPase RhoB in the early developing mouse lens.Dev Dyn 222, no. 3 (November 2001): 534–37. https://doi.org/10.1002/dvdy.1202.
Maddala R, Peng YW, Rao PV. Selective expression of the small GTPase RhoB in the early developing mouse lens. Dev Dyn. 2001 Nov;222(3):534–7.
Maddala, R., et al. “Selective expression of the small GTPase RhoB in the early developing mouse lens.Dev Dyn, vol. 222, no. 3, Nov. 2001, pp. 534–37. Pubmed, doi:10.1002/dvdy.1202.
Maddala R, Peng YW, Rao PV. Selective expression of the small GTPase RhoB in the early developing mouse lens. Dev Dyn. 2001 Nov;222(3):534–537.
Journal cover image

Published In

Dev Dyn

DOI

ISSN

1058-8388

Publication Date

November 2001

Volume

222

Issue

3

Start / End Page

534 / 537

Location

United States

Related Subject Headings

  • rhoB GTP-Binding Protein
  • Tissue Distribution
  • Reverse Transcriptase Polymerase Chain Reaction
  • Mice
  • Lens, Crystalline
  • In Vitro Techniques
  • Immunohistochemistry
  • Embryo, Mammalian
  • Developmental Biology
  • Blotting, Western