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Purification and characterization of zeta-crystallin/quinone reductase from guinea pig liver.

Publication ,  Journal Article
Rao, PV; Zigler, JS
Published in: Biochim Biophys Acta
October 27, 1992

zeta-Crystallin, a major lens protein of certain mammalian species, has recently been characterized as a novel and active NADPH:quinone oxidoreductase. Here we report the purification of this protein from guinea pig liver by utilizing sequentially: ammonium sulphate precipitation, Blue Sepharose affinity, cation exchange and hydrophobic chromatography steps. This four-step isolation procedure yielded 118-fold purification and a specific activity of 6 U/mg protein when assayed in the presence of 9,10-phenanthrenequinone. Kinetic, immunological and physical properties of this protein have been found to be identical with those of guinea pig lens zeta-crystallin. Western blot analysis using antibodies raised against zeta-crystallin peptides demonstrated the presence of substantial amounts of this protein in human liver homogenates.

Duke Scholars

Published In

Biochim Biophys Acta

ISSN

0006-3002

Publication Date

October 27, 1992

Volume

1117

Issue

3

Start / End Page

315 / 320

Location

Netherlands

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Quinone Reductases
  • Liver
  • Kinetics
  • Humans
  • Guinea Pigs
  • Crystallins
  • Chromatography, Liquid
  • Chemical Fractionation
 

Citation

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Rao, P. V., & Zigler, J. S. (1992). Purification and characterization of zeta-crystallin/quinone reductase from guinea pig liver. Biochim Biophys Acta, 1117(3), 315–320.
Rao, P. V., and J. S. Zigler. “Purification and characterization of zeta-crystallin/quinone reductase from guinea pig liver.Biochim Biophys Acta 1117, no. 3 (October 27, 1992): 315–20.
Rao PV, Zigler JS. Purification and characterization of zeta-crystallin/quinone reductase from guinea pig liver. Biochim Biophys Acta. 1992 Oct 27;1117(3):315–20.
Rao, P. V., and J. S. Zigler. “Purification and characterization of zeta-crystallin/quinone reductase from guinea pig liver.Biochim Biophys Acta, vol. 1117, no. 3, Oct. 1992, pp. 315–20.
Rao PV, Zigler JS. Purification and characterization of zeta-crystallin/quinone reductase from guinea pig liver. Biochim Biophys Acta. 1992 Oct 27;1117(3):315–320.

Published In

Biochim Biophys Acta

ISSN

0006-3002

Publication Date

October 27, 1992

Volume

1117

Issue

3

Start / End Page

315 / 320

Location

Netherlands

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Quinone Reductases
  • Liver
  • Kinetics
  • Humans
  • Guinea Pigs
  • Crystallins
  • Chromatography, Liquid
  • Chemical Fractionation