Zeta-crystallin from guinea pig lens is capable of functioning catalytically as an oxidoreductase.
Publication
, Journal Article
Rao, PV; Zigler, JS
Published in: Arch Biochem Biophys
January 1991
zeta-Crystallin, a major taxon-specific protein of the guinea pig lens, has been shown to be distantly related to the alcohol/polyol dehydrogenase family and to specifically bind NADPH. The capacity of zeta-crystallin to function catalytically was investigated in the present study. zeta-Crystallin exhibited an NADPH-dependent oxidoreductase activity with 2,6-dichlorophenolindophenol (DCIP). The NADPH:DCIP oxidoreductase activity of zeta-crystallin exhibits a linear response with increasing protein concentration, and saturation kinetics with NADPH and DCIP. This activity was abolished by heat inactivation and immunoadsorption of the protein. Dicumarol, Cibacron blue, manganese, and sulfhydryl reagents were inhibitory.
Duke Scholars
Published In
Arch Biochem Biophys
DOI
ISSN
0003-9861
Publication Date
January 1991
Volume
284
Issue
1
Start / End Page
181 / 185
Location
United States
Related Subject Headings
- Oxidoreductases
- NADP
- Molecular Weight
- Lens, Crystalline
- In Vitro Techniques
- Guinea Pigs
- Crystallins
- Biochemistry & Molecular Biology
- Animals
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Rao, P. V., & Zigler, J. S. (1991). Zeta-crystallin from guinea pig lens is capable of functioning catalytically as an oxidoreductase. Arch Biochem Biophys, 284(1), 181–185. https://doi.org/10.1016/0003-9861(91)90281-m
Rao, P. V., and J. S. Zigler. “Zeta-crystallin from guinea pig lens is capable of functioning catalytically as an oxidoreductase.” Arch Biochem Biophys 284, no. 1 (January 1991): 181–85. https://doi.org/10.1016/0003-9861(91)90281-m.
Rao PV, Zigler JS. Zeta-crystallin from guinea pig lens is capable of functioning catalytically as an oxidoreductase. Arch Biochem Biophys. 1991 Jan;284(1):181–5.
Rao, P. V., and J. S. Zigler. “Zeta-crystallin from guinea pig lens is capable of functioning catalytically as an oxidoreductase.” Arch Biochem Biophys, vol. 284, no. 1, Jan. 1991, pp. 181–85. Pubmed, doi:10.1016/0003-9861(91)90281-m.
Rao PV, Zigler JS. Zeta-crystallin from guinea pig lens is capable of functioning catalytically as an oxidoreductase. Arch Biochem Biophys. 1991 Jan;284(1):181–185.
Published In
Arch Biochem Biophys
DOI
ISSN
0003-9861
Publication Date
January 1991
Volume
284
Issue
1
Start / End Page
181 / 185
Location
United States
Related Subject Headings
- Oxidoreductases
- NADP
- Molecular Weight
- Lens, Crystalline
- In Vitro Techniques
- Guinea Pigs
- Crystallins
- Biochemistry & Molecular Biology
- Animals
- 3101 Biochemistry and cell biology