Extremely high levels of NADPH in guinea pig lens: correlation with zeta-crystallin concentration.

Journal Article (Journal Article)

Zeta-crystallin, a major "taxon-specific" protein of the guinea pig lens, specifically binds NADPH. Analysis of pyridine nucleotide levels in guinea pig lens revealed values for NADPH approximately 50-fold higher than in other lenses. Indeed to our knowledge the values reported are higher than have been observed in any tissue. A clear correlation exists between NADPH and zeta-crystallin contents of the lens both in normal guinea pigs during development and in a line of guinea pigs with a mutation in the gene for zeta-crystallin. Heterozygotes for this mutation had a 50% reduction in NADPH, while homozygotes have only about 6% of the normal level. NADP+ levels were also markedly elevated suggesting that redox cycling of the NADPH is occurring.

Full Text

Duke Authors

Cited Authors

  • Rao, PV; Zigler, JS

Published Date

  • March 30, 1990

Published In

Volume / Issue

  • 167 / 3

Start / End Page

  • 1221 - 1228

PubMed ID

  • 2322267

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/0006-291x(90)90654-6


  • eng

Conference Location

  • United States