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Development and preclinical evaluation of an alphavirus replicon vaccine for influenza.

Publication ,  Journal Article
Hubby, B; Talarico, T; Maughan, M; Reap, EA; Berglund, P; Kamrud, KI; Copp, L; Lewis, W; Cecil, C; Norberg, P; Wagner, J; Watson, A; Negri, S ...
Published in: Vaccine
November 23, 2007
Published version (DOI) Link to item

We used a propagation-defective, single-cycle, alphavirus replicon vector system to produce virus-like replicon particles (VRP) expressing the hemagglutinin (HA) and neuraminidase (NA) proteins from influenza A/Wyoming/03/2003 (H3N2). Efficient production methods were scaled to produce pilot lots of HA VRP and NA VRP and clinical lots of HA VRP. HA VRP-induced high-titered antibody responses in mice, rabbits and rhesus macaques, as measured by ELISA or hemagglutination inhibition (HI) assays, and robust cellular immune responses in mice and rhesus macaques, as measured by IFN-gamma ELISPOT. NA VRP also induced cellular immune responses in mice. A toxicology study with HA VRP and NA VRP in rabbits showed no adverse effects in any parameter. These studies support clinical testing of alphavirus replicon vaccines for influenza.

Duke Scholars

Bruce Kendall Burnett
Author Bruce Kendall Burnett Medicine, Clinical Pharmacology
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Published In

Vaccine

DOI

10.1016/j.vaccine.2007.09.038

ISSN

0264-410X

Publication Date

November 23, 2007

Volume

25

Issue

48

Start / End Page

8180 / 8189

Location

Netherlands

Related Subject Headings

  • Virology
  • Replicon
  • Rabbits
  • Neuraminidase
  • Mice
  • Macaca mulatta
  • Influenza Vaccines
  • Influenza A Virus, H3N2 Subtype
  • Immunity, Cellular
  • Hemagglutinin Glycoproteins, Influenza Virus
 

Citation

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Hubby, B., Talarico, T., Maughan, M., Reap, E. A., Berglund, P., Kamrud, K. I., … Chulay, J. D. (2007). Development and preclinical evaluation of an alphavirus replicon vaccine for influenza. Vaccine, 25(48), 8180–8189. https://doi.org/10.1016/j.vaccine.2007.09.038
Hubby, Bolyn, Todd Talarico, Maureen Maughan, Elizabeth A. Reap, Peter Berglund, Kurt I. Kamrud, Laura Copp, et al. “Development and preclinical evaluation of an alphavirus replicon vaccine for influenza.” Vaccine 25, no. 48 (November 23, 2007): 8180–89. https://doi.org/10.1016/j.vaccine.2007.09.038.
Hubby B, Talarico T, Maughan M, Reap EA, Berglund P, Kamrud KI, et al. Development and preclinical evaluation of an alphavirus replicon vaccine for influenza. Vaccine. 2007 Nov 23;25(48):8180–9.
Hubby, Bolyn, et al. “Development and preclinical evaluation of an alphavirus replicon vaccine for influenza.” Vaccine, vol. 25, no. 48, Nov. 2007, pp. 8180–89. Pubmed, doi:10.1016/j.vaccine.2007.09.038.
Hubby B, Talarico T, Maughan M, Reap EA, Berglund P, Kamrud KI, Copp L, Lewis W, Cecil C, Norberg P, Wagner J, Watson A, Negri S, Burnett BK, Graham A, Smith JF, Chulay JD. Development and preclinical evaluation of an alphavirus replicon vaccine for influenza. Vaccine. 2007 Nov 23;25(48):8180–8189.
Journal cover image

Published In

Vaccine

DOI

10.1016/j.vaccine.2007.09.038

ISSN

0264-410X

Publication Date

November 23, 2007

Volume

25

Issue

48

Start / End Page

8180 / 8189

Location

Netherlands

Related Subject Headings

  • Virology
  • Replicon
  • Rabbits
  • Neuraminidase
  • Mice
  • Macaca mulatta
  • Influenza Vaccines
  • Influenza A Virus, H3N2 Subtype
  • Immunity, Cellular
  • Hemagglutinin Glycoproteins, Influenza Virus