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Haptotactic gradients for directed cell migration: stimulation and inhibition using soluble factors.

Publication ,  Journal Article
Smith, JT; Kim, DH; Reichert, WM
Published in: Combinatorial chemistry & high throughput screening
July 2009

A recently developed technique for the measurement of cell migration on surface bound gradients was used to assay the behavior of microvascular endothelial cells on a range of fibronectin gradient slopes in the presence of soluble promoters and inhibitors of chemotaxis. Directional microvascular endothelial cell migration was shown to increase with increasing gradient slope with no significant change in cellular persistence time or random cell speed. Uniformly distributed soluble chemotactic factor in the hMEC growth media enhanced directional migration. The addition of migration-inhibiting LY294002 eliminated the directional component of cell migration at a 5 microM dosing. These experiments broaden the understanding of the directional nature of cell motion and present a reliable system for the quantitative study of cell migration in complex conditions in vitro.

Duke Scholars

Published In

Combinatorial chemistry & high throughput screening

DOI

EISSN

1875-5402

ISSN

1386-2073

Publication Date

July 2009

Volume

12

Issue

6

Start / End Page

598 / 603

Related Subject Headings

  • Organic Chemistry
  • Morpholines
  • Humans
  • Fibroblast Growth Factors
  • Enzyme Inhibitors
  • Endothelial Cells
  • Combinatorial Chemistry Techniques
  • Chromones
  • Chemotaxis
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Smith, J. T., Kim, D. H., & Reichert, W. M. (2009). Haptotactic gradients for directed cell migration: stimulation and inhibition using soluble factors. Combinatorial Chemistry & High Throughput Screening, 12(6), 598–603. https://doi.org/10.2174/138620709788681871
Smith, Jason T., Donghwan H. Kim, and William M. Reichert. “Haptotactic gradients for directed cell migration: stimulation and inhibition using soluble factors.Combinatorial Chemistry & High Throughput Screening 12, no. 6 (July 2009): 598–603. https://doi.org/10.2174/138620709788681871.
Smith JT, Kim DH, Reichert WM. Haptotactic gradients for directed cell migration: stimulation and inhibition using soluble factors. Combinatorial chemistry & high throughput screening. 2009 Jul;12(6):598–603.
Smith, Jason T., et al. “Haptotactic gradients for directed cell migration: stimulation and inhibition using soluble factors.Combinatorial Chemistry & High Throughput Screening, vol. 12, no. 6, July 2009, pp. 598–603. Epmc, doi:10.2174/138620709788681871.
Smith JT, Kim DH, Reichert WM. Haptotactic gradients for directed cell migration: stimulation and inhibition using soluble factors. Combinatorial chemistry & high throughput screening. 2009 Jul;12(6):598–603.

Published In

Combinatorial chemistry & high throughput screening

DOI

EISSN

1875-5402

ISSN

1386-2073

Publication Date

July 2009

Volume

12

Issue

6

Start / End Page

598 / 603

Related Subject Headings

  • Organic Chemistry
  • Morpholines
  • Humans
  • Fibroblast Growth Factors
  • Enzyme Inhibitors
  • Endothelial Cells
  • Combinatorial Chemistry Techniques
  • Chromones
  • Chemotaxis
  • Cells, Cultured