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Cytokine profiling using monocytes/macrophages cultured on common biomaterials with a range of surface chemistries.

Publication ,  Journal Article
Schutte, RJ; Parisi-Amon, A; Reichert, WM
Published in: Journal of biomedical materials research. Part A
January 2009

Cytokines, chemokines, and growth factors were assayed from the supernatants of monocytes and macrophages cultured on common biomaterials with a range of surface chemistries. TNF-alpha, MCP-1, MIP-1alpha, IL-8, IL-6, IL-1beta, VEGF, IL-1ra, and IL-10 were measured from monocyte/macrophage cultures at different stages of activation and differentiation seeded onto polyethylene, polyurethane, expanded polytetrafluoroethylene, polymethyl methacrylate, and a hydrogel copolymer of 2-hydroxyethyl methacrylate, 1-vinyl-2-pyrrolidinone, and polyethylene glycol acrylate in tissue culture polystyrene (TCPS) plates. Empty TCPS wells and organo-tin polyvinyl chloride served as "blanks" and positive controls, respectively. Results showed an overall increase in cytokine, chemokine, and growth factor production as monocytes are activated or differentiated into macrophages and that proinflammatory and anti-wound healing cytokines and chemokines dominate this profile. However, cytokine production was only modestly affected by the surface chemistry of these four stable and noncytotoxic biomaterials.

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Published In

Journal of biomedical materials research. Part A

DOI

EISSN

1552-4965

ISSN

1549-3296

Publication Date

January 2009

Volume

88

Issue

1

Start / End Page

128 / 139

Related Subject Headings

  • Surface Properties
  • Polymers
  • Monocytes
  • Methods
  • Macrophages
  • Macrophage Activation
  • Intercellular Signaling Peptides and Proteins
  • Humans
  • Cytokines
  • Chemokines
 

Citation

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Schutte, R. J., Parisi-Amon, A., & Reichert, W. M. (2009). Cytokine profiling using monocytes/macrophages cultured on common biomaterials with a range of surface chemistries. Journal of Biomedical Materials Research. Part A, 88(1), 128–139. https://doi.org/10.1002/jbm.a.31863
Schutte, Robert J., Andreina Parisi-Amon, and W Monty Reichert. “Cytokine profiling using monocytes/macrophages cultured on common biomaterials with a range of surface chemistries.Journal of Biomedical Materials Research. Part A 88, no. 1 (January 2009): 128–39. https://doi.org/10.1002/jbm.a.31863.
Schutte RJ, Parisi-Amon A, Reichert WM. Cytokine profiling using monocytes/macrophages cultured on common biomaterials with a range of surface chemistries. Journal of biomedical materials research Part A. 2009 Jan;88(1):128–39.
Schutte, Robert J., et al. “Cytokine profiling using monocytes/macrophages cultured on common biomaterials with a range of surface chemistries.Journal of Biomedical Materials Research. Part A, vol. 88, no. 1, Jan. 2009, pp. 128–39. Epmc, doi:10.1002/jbm.a.31863.
Schutte RJ, Parisi-Amon A, Reichert WM. Cytokine profiling using monocytes/macrophages cultured on common biomaterials with a range of surface chemistries. Journal of biomedical materials research Part A. 2009 Jan;88(1):128–139.
Journal cover image

Published In

Journal of biomedical materials research. Part A

DOI

EISSN

1552-4965

ISSN

1549-3296

Publication Date

January 2009

Volume

88

Issue

1

Start / End Page

128 / 139

Related Subject Headings

  • Surface Properties
  • Polymers
  • Monocytes
  • Methods
  • Macrophages
  • Macrophage Activation
  • Intercellular Signaling Peptides and Proteins
  • Humans
  • Cytokines
  • Chemokines