Cytokine profiling using monocytes/macrophages cultured on common biomaterials with a range of surface chemistries.
Journal Article (Journal Article)
Cytokines, chemokines, and growth factors were assayed from the supernatants of monocytes and macrophages cultured on common biomaterials with a range of surface chemistries. TNF-alpha, MCP-1, MIP-1alpha, IL-8, IL-6, IL-1beta, VEGF, IL-1ra, and IL-10 were measured from monocyte/macrophage cultures at different stages of activation and differentiation seeded onto polyethylene, polyurethane, expanded polytetrafluoroethylene, polymethyl methacrylate, and a hydrogel copolymer of 2-hydroxyethyl methacrylate, 1-vinyl-2-pyrrolidinone, and polyethylene glycol acrylate in tissue culture polystyrene (TCPS) plates. Empty TCPS wells and organo-tin polyvinyl chloride served as "blanks" and positive controls, respectively. Results showed an overall increase in cytokine, chemokine, and growth factor production as monocytes are activated or differentiated into macrophages and that proinflammatory and anti-wound healing cytokines and chemokines dominate this profile. However, cytokine production was only modestly affected by the surface chemistry of these four stable and noncytotoxic biomaterials.
Full Text
Duke Authors
Cited Authors
- Schutte, RJ; Parisi-Amon, A; Reichert, WM
Published Date
- January 2009
Published In
Volume / Issue
- 88 / 1
Start / End Page
- 128 - 139
PubMed ID
- 18260130
Pubmed Central ID
- PMC4070304
Electronic International Standard Serial Number (EISSN)
- 1552-4965
International Standard Serial Number (ISSN)
- 1549-3296
Digital Object Identifier (DOI)
- 10.1002/jbm.a.31863
Language
- eng