Cytokine profiling using monocytes/macrophages cultured on common biomaterials with a range of surface chemistries.

Journal Article

Cytokines, chemokines, and growth factors were assayed from the supernatants of monocytes and macrophages cultured on common biomaterials with a range of surface chemistries. TNF-alpha, MCP-1, MIP-1alpha, IL-8, IL-6, IL-1beta, VEGF, IL-1ra, and IL-10 were measured from monocyte/macrophage cultures at different stages of activation and differentiation seeded onto polyethylene, polyurethane, expanded polytetrafluoroethylene, polymethyl methacrylate, and a hydrogel copolymer of 2-hydroxyethyl methacrylate, 1-vinyl-2-pyrrolidinone, and polyethylene glycol acrylate in tissue culture polystyrene (TCPS) plates. Empty TCPS wells and organo-tin polyvinyl chloride served as "blanks" and positive controls, respectively. Results showed an overall increase in cytokine, chemokine, and growth factor production as monocytes are activated or differentiated into macrophages and that proinflammatory and anti-wound healing cytokines and chemokines dominate this profile. However, cytokine production was only modestly affected by the surface chemistry of these four stable and noncytotoxic biomaterials.

Full Text

Duke Authors

Cited Authors

  • Schutte, RJ; Parisi-Amon, A; Reichert, WM

Published Date

  • January 2009

Published In

Volume / Issue

  • 88 / 1

Start / End Page

  • 128 - 139

PubMed ID

  • 18260130

Electronic International Standard Serial Number (EISSN)

  • 1552-4965

Digital Object Identifier (DOI)

  • 10.1002/jbm.a.31863

Language

  • eng

Conference Location

  • United States