Directed cell migration on fibronectin gradients: effect of gradient slope.

Journal Article (Journal Article)

The migration of human microvascular endothelial cells (hMEC) was measured on a range of fibronectin gradient slopes. hMEC drift speed increased with increasing gradient slope with no concurrent change in cellular persistence time or random cell speed. The frequency of discrete cellular motion in the gradient direction increased with gradient slope. Morphological polarization of cells on the gradients is also characterized and correlated with cellular drift speed. These experiments present the first demonstration of cellular response to changing haptotactic gradient slope using an in vitro system for the quantitative study of cell migration.

Full Text

Duke Authors

Cited Authors

  • Smith, JT; Elkin, JT; Reichert, WM

Published Date

  • August 2006

Published In

Volume / Issue

  • 312 / 13

Start / End Page

  • 2424 - 2432

PubMed ID

  • 16730349

Electronic International Standard Serial Number (EISSN)

  • 1090-2422

International Standard Serial Number (ISSN)

  • 0014-4827

Digital Object Identifier (DOI)

  • 10.1016/j.yexcr.2006.04.005


  • eng