Migration assay to measure cellular response to signalling gradients
The use of a modified Teflon fence assay to track the movement of endothelical cells in response to a diffusion generated signalling gradient of fibronectin was discussed. The substrates were generated from self-assembled monolayers of alkanethoils on optically transparent Au (silver) coated glass slides. Bovine aortic cells (BAEC) were grown to a confluent monolayer behind a Teflon fence positioned at 20% of the maximum fibronectin concentration on the gradients. Cells were cultured in Dulbecccco's modified eagles medium supplemented with 10% serum and 2% penicillin/streptomycin during seeding. It was observed that the gradient and the control substrates had velocity in the X direction over the first 9 hours of the migration assay.