Autonomous programmable nanorobotic devices using DNAzymes


Journal Article

A major challenge in nanoscience is the design of synthetic molecular devices that run autonomously and are programmable. DNA-based synthetic molecular devices have the advantage of being relatively simple to design and engineer, due to the predictable secondary structure of DNA nanostructures and the well-established biochemistry used to manipulate DNA nanostructures. We present the design of a class of DNAzyme based molecular devices that are autonomous, programmable, and further require no protein enzymes. The basic principle involved is inspired by a simple but ingenious molecular device due to Mao et al [25]. Our DNAzyme based designs include (1) a finite state automata device, DNAzyme FSA that executes finite state transitions using DNAzymes, (2) extensions to it including probabilistic automata and non-deterministic automata, (3) its application as a DNAzyme router for programmable routing of nanostructures on a 2D DNA addressable lattice, and (4) a medical-related application, DNAzyme doctor that provide transduction of nucleic acid expression: it can be programmed to respond to the underexpression or overexpression of various strands of RNA, with a response by release of an RNA. © 2008 Springer-Verlag Berlin Heidelberg.

Full Text

Duke Authors

Cited Authors

  • Reif, JH; Sahu, S

Published Date

  • August 27, 2008

Published In

Volume / Issue

  • 4848 LNCS /

Start / End Page

  • 66 - 78

Electronic International Standard Serial Number (EISSN)

  • 1611-3349

International Standard Serial Number (ISSN)

  • 0302-9743

Digital Object Identifier (DOI)

  • 10.1007/978-3-540-77962-9_7

Citation Source

  • Scopus