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Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia.

Publication ,  Journal Article
Crump, JA; Tuohy, MJ; Morrissey, AB; Ramadhani, HO; Njau, BN; Maro, VP; Reller, LB; Procop, GW
Published in: J Clin Microbiol
January 2012

To investigate the performance of a nucleic acid amplification test (NAAT) for the diagnosis of Mycobacterium tuberculosis bacteremia, 5-ml aliquots of blood were inoculated into bioMérieux mycobacterial (MB) bottles and incubated, and 5-ml aliquots of blood were extracted and tested by real-time PCR. Of 25 samples from patients with M. tuberculosis bacteremia, 9 (36.0%) were positive and 1 (1.5%) of 66 control samples was positive by NAAT. The NAAT shows promise, but modifications should focus on improving sensitivity.

Duke Scholars

Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

January 2012

Volume

50

Issue

1

Start / End Page

138 / 141

Location

United States

Related Subject Headings

  • Young Adult
  • Tuberculosis
  • Specimen Handling
  • Sensitivity and Specificity
  • Real-Time Polymerase Chain Reaction
  • Mycobacterium tuberculosis
  • Middle Aged
  • Microbiology
  • Humans
  • Blood
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Crump, J. A., Tuohy, M. J., Morrissey, A. B., Ramadhani, H. O., Njau, B. N., Maro, V. P., … Procop, G. W. (2012). Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia. J Clin Microbiol, 50(1), 138–141. https://doi.org/10.1128/JCM.05963-11
Crump, John A., Marion J. Tuohy, Anne B. Morrissey, Habib O. Ramadhani, Boniface N. Njau, Venance P. Maro, L Barth Reller, and Gary W. Procop. “Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia.J Clin Microbiol 50, no. 1 (January 2012): 138–41. https://doi.org/10.1128/JCM.05963-11.
Crump JA, Tuohy MJ, Morrissey AB, Ramadhani HO, Njau BN, Maro VP, et al. Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia. J Clin Microbiol. 2012 Jan;50(1):138–41.
Crump, John A., et al. “Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia.J Clin Microbiol, vol. 50, no. 1, Jan. 2012, pp. 138–41. Pubmed, doi:10.1128/JCM.05963-11.
Crump JA, Tuohy MJ, Morrissey AB, Ramadhani HO, Njau BN, Maro VP, Reller LB, Procop GW. Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia. J Clin Microbiol. 2012 Jan;50(1):138–141.

Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

January 2012

Volume

50

Issue

1

Start / End Page

138 / 141

Location

United States

Related Subject Headings

  • Young Adult
  • Tuberculosis
  • Specimen Handling
  • Sensitivity and Specificity
  • Real-Time Polymerase Chain Reaction
  • Mycobacterium tuberculosis
  • Middle Aged
  • Microbiology
  • Humans
  • Blood