Performance of nucleic acid amplification following extraction of 5 milliliters of whole blood for diagnosis of Mycobacterium tuberculosis bacteremia.

Journal Article (Journal Article)

To investigate the performance of a nucleic acid amplification test (NAAT) for the diagnosis of Mycobacterium tuberculosis bacteremia, 5-ml aliquots of blood were inoculated into bioMérieux mycobacterial (MB) bottles and incubated, and 5-ml aliquots of blood were extracted and tested by real-time PCR. Of 25 samples from patients with M. tuberculosis bacteremia, 9 (36.0%) were positive and 1 (1.5%) of 66 control samples was positive by NAAT. The NAAT shows promise, but modifications should focus on improving sensitivity.

Full Text

Duke Authors

Cited Authors

  • Crump, JA; Tuohy, MJ; Morrissey, AB; Ramadhani, HO; Njau, BN; Maro, VP; Reller, LB; Procop, GW

Published Date

  • January 2012

Published In

Volume / Issue

  • 50 / 1

Start / End Page

  • 138 - 141

PubMed ID

  • 22031703

Pubmed Central ID

  • 22031703

Electronic International Standard Serial Number (EISSN)

  • 1098-660X

Digital Object Identifier (DOI)

  • 10.1128/JCM.05963-11


  • eng

Conference Location

  • United States