Evaluation of modified trypticase soy broth versus supplemented peptone broth in the detection of bacteremia and fungemia.

Journal Article (Journal Article)

In vitro, 1.2% gelatin counteracts the inhibition of growth of bacterial species by sodium polyanetholsulfonate in blood culture media. Additionally, 1% yeast extract has been used to promote bacterial growth. We compared the performance of supplemented peptone broth and Trypticase soy broth, both of which contained sodium polyanetholsulfonate, gelatin and yeast extract. Trypticase soy broth with gelatin and yeast extract inhibited (p less than 0.001) and delayed growth, especially of gram-positive (p less than 0.01) and gram-negative (p less than 0.005) anaerobic bacteria. Although the recovery of organisms usually inhibited by sodium polyanetholsulfonate was similar in supplemented peptone and Trypticase soy broths, supplemented peptone broth clearly was superior in the recovery of other organisms commonly found in blood cultures.

Full Text

Duke Authors

Cited Authors

  • Reimer, L; Reller, B; Wang, WL; Mirrett, S

Published Date

  • June 1988

Published In

Volume / Issue

  • 7 / 3

Start / End Page

  • 384 - 387

PubMed ID

  • 2970385

International Standard Serial Number (ISSN)

  • 0934-9723

Digital Object Identifier (DOI)

  • 10.1007/BF01962342


  • eng

Conference Location

  • Germany