Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 A resolution.

Published

Journal Article

The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.

Full Text

Duke Authors

Cited Authors

  • Kelley, L-LC; Dillard, BD; Tempel, W; Chen, L; Shaw, N; Lee, D; Newton, MG; Sugar, FJ; Jenney, FE; Lee, HS; Shah, C; Poole, FL; Adams, MWW; Richardson, JS; Richardson, DC; Liu, Z-J; Wang, B-C; Rose, J

Published Date

  • July 2007

Published In

Volume / Issue

  • 63 / Pt 7

Start / End Page

  • 549 - 552

PubMed ID

  • 17620707

Pubmed Central ID

  • 17620707

Electronic International Standard Serial Number (EISSN)

  • 1744-3091

Digital Object Identifier (DOI)

  • 10.1107/S1744309107024049

Language

  • eng