Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 A resolution.
The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.
Kelley, L-LC; Dillard, BD; Tempel, W; Chen, L; Shaw, N; Lee, D; Newton, MG; Sugar, FJ; Jenney, FE; Lee, HS; Shah, C; Poole, FL; Adams, MWW; Richardson, JS; Richardson, DC; Liu, Z-J; Wang, B-C; Rose, J
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