Structure of the hypothetical protein PF0899 from Pyrococcus furiosus at 1.85 A resolution.
Journal Article (Journal Article)
The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.
Full Text
Duke Authors
Cited Authors
- Kelley, L-LC; Dillard, BD; Tempel, W; Chen, L; Shaw, N; Lee, D; Newton, MG; Sugar, FJ; Jenney, FE; Lee, HS; Shah, C; Poole, FL; Adams, MWW; Richardson, JS; Richardson, DC; Liu, Z-J; Wang, B-C; Rose, J
Published Date
- July 1, 2007
Published In
Volume / Issue
- 63 / Pt 7
Start / End Page
- 549 - 552
PubMed ID
- 17620707
Pubmed Central ID
- PMC2335137
Electronic International Standard Serial Number (EISSN)
- 1744-3091
Digital Object Identifier (DOI)
- 10.1107/S1744309107024049
Language
- eng
Conference Location
- England