Molecular visualization is used by instructors to enhance the student's learning, although few assessments are available to validate this belief. The first performance assessment showed that lecture and lab effectively communicated several core concepts in protein biochemistry. The purpose of the current study was to build on White's study and investigate what biochemistry students learned from the process of actually constructing annotated graphic images of biological macromolecule using experimentally solved coordinate sets from the protein data bank along with the related primary literature. The survey data showed a positive effect on student attitudes about learning various aspects of protein structure, especially higher-order structural concepts.