The high-throughput protein-to-structure pipeline at SECSG.

Published

Journal Article

Using a high degree of automation, the crystallography core at the Southeast Collaboratory for Structural Genomics (SECSG) has developed a high-throughput protein-to-structure pipeline. Various robots and automation procedures have been adopted and integrated into a pipeline that is capable of screening 40 proteins for crystallization and solving four protein structures per week. This pipeline is composed of three major units: crystallization, structure determination/validation and crystallomics. Coupled with the protein-production cores at SECSG, the protein-to-structure pipeline provides a two-tiered approach for protein production at SECSG. In tier 1, all protein samples supplied by the protein-production cores pass through the pipeline using standard crystallization screening and optimization procedures. The protein targets that failed to yield diffraction-quality crystals (resolution better than 3.0 A) become tier 2 or salvaging targets. The goal of tier 2 target salvaging, carried out by the crystallomics core, is to produce the target proteins with increased purity and homogeneity, which would render them more likely to yield well diffracting crystals. This is performed by alternative purification procedures and/or the introduction of chemical modifications to the proteins (such as tag removal, methylation, surface mutagenesis, selenomethionine labelling etc.). Details of the various procedures in the pipeline for protein crystallization, target salvaging, data collection/processing and high-throughput structure determination/validation, as well as some examples, are described.

Full Text

Duke Authors

Cited Authors

  • Liu, Z-J; Tempel, W; Ng, JD; Lin, D; Shah, AK; Chen, L; Horanyi, PS; Habel, JE; Kataeva, IA; Xu, H; Yang, H; Chang, JC; Huang, L; Chang, S-H; Zhou, W; Lee, D; Praissman, JL; Zhang, H; Newton, MG; Rose, JP; Richardson, JS; Richardson, DC; Wang, B-C

Published Date

  • June 2005

Published In

Volume / Issue

  • 61 / Pt 6

Start / End Page

  • 679 - 684

PubMed ID

  • 15930619

Pubmed Central ID

  • 15930619

Electronic International Standard Serial Number (EISSN)

  • 1399-0047

International Standard Serial Number (ISSN)

  • 0907-4449

Digital Object Identifier (DOI)

  • 10.1107/s0907444905013132

Language

  • eng