Evidence for the involvement of cyclic AMP in the pheromonal modulation of barnacle settlement


Journal Article

The involvement of cyclic AMP in the settlement of the cypris larva of Balanus amphitrite amphitrite Darwin has been examined through the use of compounds that affect intracellular cyclic AMP levels. The activation of adenylate cyclase with forskolin, and the inhibition of phosphodiesterase with 3-isobutyl-1-methylxanthine, caffeine and theophylline, significantly increased the settlement of cyprids. Although the analogue dibutyryl cyclic AMP appeared to increase settlement, the effect was not significant. No marked increase in settlement resulted from the incubation of cyprids with dibutyryl cyclic GMP, 8-(4-chlorophenylthio) (CPT) cyclic AMP or papaverine (a phosphodiesterase inhibitor). Miconazole nitrate, an adenylate cyclase inhibitor, prevented settlement, but this effect appeared to be physico-chemical rather than pharmacological. Radioimmunoassay did not clearly show whether cyclic AMP levels changed following exposure of cyprids to a pulse of crude barnacle extract. However, exposure to forskolin significantly increased the cyclic AMP titre of cyprids. We conclude that compounds that alter intracellular cyclic AMP levels alter normal patterns of cyprid settlement. Whether this is because of an alteration in signal transduction is unclear.

Full Text

Duke Authors

Cited Authors

  • Clare, A; Thomas, R; Rittschof, D

Published Date

  • January 1995

Published In

Volume / Issue

  • 198 / Pt 3

Start / End Page

  • 655 - 664

PubMed ID

  • 9318389

Pubmed Central ID

  • 9318389

Electronic International Standard Serial Number (EISSN)

  • 1477-9145

International Standard Serial Number (ISSN)

  • 0022-0949


  • eng